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Literature summary extracted from

  • Pawelek, P.D.; MacKenzie, R.E.
    Methenyltetrahydrofolate cyclohydrolase is rate limiting for the enzymatic conversion of 10-formyltetrahydrofolate to 5,10-methylenetetrahydrofolate in bifunctional dehydrogenase-cyclohydrolase enzymes (1998), Biochemistry, 37, 1109-1115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.9 cDNA encoding bifunctional enzyme cloned and expressed in Escherichia coli K38 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.008
-
 5,10-methylenetetrahydrofolate deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 0.009
-
 5,10-methylenetetrahydrofolate NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 0.01
-
 5,10-methylenetetrahydrofolate bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme Photobacterium phosphoreum
1.5.1.5 0.012
-
 5,10-methylenetetrahydrofolate NAD+-M2+-phopsphate-dependent mitochondrial dehydrogenase-cyclohydrolase enzyme Homo sapiens
1.5.1.5 0.022
-
 NADP+ NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 0.038
-
 NADP+ bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme Photobacterium phosphoreum
1.5.1.5 0.507
-
 NAD+ NAD+-M2+-phopsphate-dependent mitochondrial dehydrogenase-cyclohydrolase enzyme Homo sapiens
3.5.4.9 0.056
-
 10-formyltetrahydrofolate
-
 Photobacterium phosphoreum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.9 cytoplasm
-
 Mus musculus 5737
-
3.5.4.9 cytoplasm
-
 Homo sapiens 5737
-
3.5.4.9 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.9 34000
-
 SDS-PAGE Mus musculus
3.5.4.9 100000
-
-
 Sus scrofa
3.5.4.9 100000
-
 SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Photobacterium phosphoreum
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 10-formyltetrahydrofolate Homo sapiens
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate Sus scrofa
-
 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Mus musculus
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Sus scrofa
-
 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Photobacterium phosphoreum
-
 10-formyltetrahydrofolate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Homo sapiens
-
-
-
1.5.1.5 Photobacterium phosphoreum
-
-
-
3.5.4.9 Homo sapiens
-
 human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme
-
3.5.4.9 Mus musculus
-
 Ehrlich ascites cells
-
3.5.4.9 Photobacterium phosphoreum
-
 marine bioluminescent bacterium, bifunctional enzyme
-
3.5.4.9 Saccharomyces cerevisiae
-
 yeast, trifunctional enzyme
-
3.5.4.9 Sus scrofa
-
 pig, trifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.9 recombinant protein expressed in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.9 44
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Photobacterium phosphoreum 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ deuterated folate substrate also Homo sapiens 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 10-formyltetrahydrofolate
-
 Homo sapiens 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 10-formyltetrahydrofolate
-
 Sus scrofa 5,10-methenyltetrahydrofolate + H2O
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Mus musculus 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Sus scrofa 10-formyltetrahydrofolate
-
 r
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Photobacterium phosphoreum 10-formyltetrahydrofolate
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.4.9 dimer
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.5 3.2
-
 5,10-methylenetetrahydrofolate deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 3.8
-
 NADP+ substrate constant: methylenetetrahydrofolate, deuterated, NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 9.4
-
 5,10-methylenetetrahydrofolate NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens
1.5.1.5 10
-
 NADP+ NADP+-dependent bifunctional dehydrogenase-cyclohydrolase domain of the cytoplasmic trifunctional enzyme Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Homo sapiens
1.5.1.5 NADP+
-
 Photobacterium phosphoreum