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Literature summary extracted from
- Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase: a multifunctional protein from porcine liver (1980), Methods Enzymol., 66, 609-615.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.5.1.5 | relatively labile, stabilized with phosphate, glycerol and NADP+ | Sus scrofa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.018 | - |
NADP+ | - |
Sus scrofa |  |
| 1.5.1.5 | 0.022 | - |
5,10-methylenetetrahydrofolate | - |
Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 33000 | - |
x * 33000, fragment has methylenetetrahydrofolate dehadrogenase and methenyltetrahydrofolate cyclohydrase activity, proteolysis, SDS-PAGE | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 61 | - |
- |
Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.1.5 | 0-4°C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Sus scrofa | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.5 | ? | x * 33000, fragment has methylenetetrahydrofolate dehadrogenase and methenyltetrahydrofolate cyclohydrase activity, proteolysis, SDS-PAGE | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NADP+ | - |
Sus scrofa | |
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