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Literature summary extracted from

  • Yeh, Y.C.; Greenberg, D.M.
    Purification and properties of N5, N10-Methylenetetra-hydrofolate dehydrogenase of calf thymus (1965), Biochim. Biophys. Acta, 105, 279-291.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.5 highly instable, partial protection against inactivation by thiol compounds, glycerol, serum albumin and EDTA, freezing inactivates purified enzyme Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 Ca2+
-
 Bos taurus
1.5.1.5 formaldehyde above 0.4 mM Bos taurus
1.5.1.5 Mg2+
-
 Bos taurus
1.5.1.5 N-ethylmaleimide
-
 Bos taurus
1.5.1.5 p-chloromercuribenzoate inhibition reversible by dimercaptopropanol Bos taurus
1.5.1.5 phenacyl bromide
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.022
-
 NADP+
-
 Bos taurus
1.5.1.5 0.08
-
 5,10-methylenetetrahydrofolate
-
 Bos taurus
1.5.1.5 0.17
-
 5,10-methylenetetrahydrofolate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.5 K+ and other monovalent metal cations Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Bos taurus
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Bos taurus
-
 young calves
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.5 thymus
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
1.5.1.5 -17°C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol, Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Bos taurus 5,10-methenyltetrahydrofolate + NADPH + H+
-
 r
1.5.1.5 5,10-methylenetetrahydropteroyltriglutamate + NADP+
-
 Bos taurus 5,10-methenyltetrahydropteroyltriglutamate + NADPH
-
 ?
1.5.1.5 DL-tetrahydropteroyl-L-aspartate + NADP+
-
 Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.5 39.5
-
 potassium phosphate buffer, pH 7.8 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 6.5
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Bos taurus