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Literature summary extracted from
- 5,10-Methylenetetrahydrofolic dehydrogenase from bakers'yeast (1962), J. Biol. Chem., 237, 1982-1988.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | 2,3-Dimercaptopropanol | the most effective thiol | Saccharomyces cerevisiae |  |
| 1.5.1.5 | 2-mercaptoethanol | - |
Saccharomyces cerevisiae | |
| 1.5.1.5 | cysteine | - |
Saccharomyces cerevisiae | |
| 1.5.1.5 | formaldehyde | maximal activity at 0.3 mM | Saccharomyces cerevisiae | |
| 1.5.1.5 | glutathione | - |
Saccharomyces cerevisiae | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.5.1.5 | frozen stable for 12 days, loss of activity upon repeated freezing and thawing | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | Borate | inhibition reversible by potassium | Saccharomyces cerevisiae | |
| 1.5.1.5 | Ca2+ | - |
Saccharomyces cerevisiae | |
| 1.5.1.5 | formaldehyde | 50% inhibition at 2.5 mM | Saccharomyces cerevisiae | |
| 1.5.1.5 | Mg2+ | - |
Saccharomyces cerevisiae | |
| 1.5.1.5 | p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.037 | - |
5,10-methylenetetrahydrofolate | - |
Saccharomyces cerevisiae | |
| 1.5.1.5 | 0.69 | - |
NADP+ | - |
Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | K+ | and other monovalent metal cations | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | Saccharomyces cerevisiae | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 259 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Saccharomyces cerevisiae | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 37 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 37 | - |
30 min stable, destroyed in 3 min at 60°C | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 7.4 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NADP+ | - |
Saccharomyces cerevisiae | |
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