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Literature summary extracted from
- Purification and characterization of thermostable 5,10-methylenetetrahydrofolate dehydrogenase from Clostridium thermoaceticum (1973), J. Biol. Chem., 248, 403-408.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NAD+ | - |
Moorella thermoacetica |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.03 | - |
5,10-methylenetetrahydrofolate | - |
Moorella thermoacetica | |
| 1.5.1.5 | 0.09 | - |
NADP+ | - |
Moorella thermoacetica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 50000 | - |
equilibrium ultracentrifugation | Moorella thermoacetica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | Moorella thermoacetica | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.5 | Moorella thermoacetica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.5 | - |
Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 362 | - |
- |
Moorella thermoacetica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.5.1.5 | -20°C, stable for at least 2 months at diluted protein concentration | Moorella thermoacetica |
| 1.5.1.5 | 4°C, stable for at least 2 months | Moorella thermoacetica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Moorella thermoacetica | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.5 | dimer | - |
Moorella thermoacetica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 64 | - |
above | Moorella thermoacetica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.5 | 37 | 50 | 30 min | Moorella thermoacetica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.5 | NADP+ | - |
Moorella thermoacetica | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.5 | 0.2 | - |
NAD+ | - |
Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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