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Literature summary extracted from

  • Ljungdahl, L.G.; O'Brien, W.E.; Moore, M.R.; Liu, M.T.
    Methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum and methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase (combined) from Clostridium thermoaceticum (1980), Methods Enzymol., 66, 599-609.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.5 freezing inactivates Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.09
-
 NADP+
-
 Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.5 30000
-
 2 * 30000, SDS-PAGE Moorella thermoacetica
1.5.1.5 55000
-
 methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined, equilibrium ultracentrifugation Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Moorella thermoacetica
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase combined Moorella thermoacetica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.5 310
-
 assays at 37°C Moorella thermoacetica
1.5.1.5 720
-
 at 60°C growth temperature Moorella thermoacetica

Storage Stability

EC Number Storage Stability Organism
1.5.1.5 4°C, stable for at least a month Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Moorella thermoacetica 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.5 dimer 2 * 30000, SDS-PAGE Moorella thermoacetica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.5 64
-
 above Moorella thermoacetica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 7
-
-
 Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Moorella thermoacetica