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Literature summary extracted from

  • Gamarro, F.; Yu, P.L.; Zhao, J.; Edman, U.; Greene, P.J.; Santi, D.
    Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme (1995), Mol. Biochem. Parasitol., 72, 11-22.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 KCl 2.5-3fold increase in activity at 0.1 M Trypanosoma brucei
1.5.1.3 NaCl 2.5-3fold increase in activity at 0.1 M Trypanosoma brucei
1.5.1.3 Urea stimulates 1.5fold at 0.75-1.5 M Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Trypanosoma brucei
1.5.1.3 pyrimethamine
-
 Trypanosoma brucei
1.5.1.3 trimethoprim
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0006
-
 7,8-dihydrofolate recombinant bifunctional enzyme Trypanosoma brucei
1.5.1.3 0.0009
-
 NADPH recombinant bifunctional enzyme Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Trypanosoma brucei
-
 trimethoprim-resistant strain
-
1.5.1.3 Trypanosoma brucei
-
 bifuntional enzyme with dihydrofolate reductase and thymidylate synthase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 recombinant bifunctional protein from Escherichia coli Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 12
-
 recombinant bifunctional enzyme Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Trypanosoma brucei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR-TS
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 5
-
 7,8-dihydrofolate recombinant bifunctional enzyme Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 5 8 broad maximum, recombinant bifunctional enzyme Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Trypanosoma brucei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 additional information
-
 additional information overview Trypanosoma brucei
1.5.1.3 0.00000023
-
 methotrexate recombinant bifunctional enzyme Trypanosoma brucei
1.5.1.3 0.00000028
-
 pyrimethamine recombinant bifunctional enzyme Trypanosoma brucei