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Literature summary extracted from

  • Fan, Y.X.; Wang, B.; Jing, G.Z.
    Soluble expression in Escherichia coli, one-step purification, and characterization of Chinese hamster dihydrofolate reductase (1997), Protein Expr. Purif., 9, 246-252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 guanidinium hydrochloride recombinant enzyme, 2.5fold activation at 0.5 M Cricetulus griseus
1.5.1.3 Urea recombinant enzyme, 4fold activation at 3 M Cricetulus griseus

Application

EC Number Application Comment Organism
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Cricetulus griseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 overexpression in Escherichia coli, regulation by temperature-sensitive repressor of the promotor Cricetulus griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.00048
-
 7,8-dihydrofolate recombinant enzyme Cricetulus griseus
1.5.1.3 0.00133
-
 NADPH recombinant enzyme Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.3 soluble recombinant enzyme from Escherichia coli Cricetulus griseus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 23000
-
 recombinant enzyme from E. coli, SDS-PAGE and DNA-sequence determination Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Cricetulus griseus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 4.7
-
 purified, recombinant enzyme Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH enzyme plays important role in nucleotide biosynthesis Cricetulus griseus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 20
-
 recombinant enzyme, assay at Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 0.65
-
 7,8-dihydrofolate recombinant enzyme Cricetulus griseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0000014
-
 methotrexate recombinant enzyme Cricetulus griseus