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Literature summary extracted from

  • Kaufman, B.T.; Gardiner, R.C.
    Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver (1966), J. Biol. Chem., 241, 1319-1328.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 N5-formyltetrahydrofolate, NADP+ and NADPH stabilize and protect against heat inactivation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 aminopterin
-
 Gallus gallus
1.5.1.3 citrate in acid pH range: inhibitor of reduction of 7,8-dihydrofolate but not folate Gallus gallus
1.5.1.3 NADP+
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.00015
-
 7,8-dihydrofolate with NADPH Gallus gallus
1.5.1.3 0.0067
-
 folate
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 23000
-
 sedimentation equilibrium studies, sucrose density gradient experiments, aminopterin titration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 liver
-
 Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 -60°C, 0.02 mM NADPH, 3 months, frequent freezing and thawing, stable Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 60
-
 half-life: 2 min Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 5
-
 7,8-dihydrofolate pH 7.4 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 3.8
-
 folate + NADPH Gallus gallus
1.5.1.3 4
-
 7,8-dihydrofolate + NADPH, 2 optima: pH 4 and pH 7.4 Gallus gallus
1.5.1.3 5.2 5.5 7,8-dihydrofolate + NADH Gallus gallus
1.5.1.3 7.4
-
 7,8-dihydrofolate + NADPH, 2 optima: pH 4.0 and pH 7.4 Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.3 6 8 maximal stability Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADH chicken liver: at neutral pH specific for NADPH and 7,8-dihydrofolate, at acid pH: NADH + 7,8-dihydrofolate and folate + NADPH Gallus gallus
1.5.1.3 NADPH
-
 Gallus gallus