Literature summary extracted from

Haber, D.A.; Beverley, S.M.; Kiely, M.L.; Schimke, R.T.
Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts (1981), J. Biol. Chem., 256, 9501-9510.

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.5.1.3	additional information	selection of cell line with altered enzyme due to decreased methotrexate-sensitivity	Mus musculus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.1.3	methasquin	-	Mus musculus
1.5.1.3	methotrexate	wild-type	Mus musculus
1.5.1.3	pyrimethamine	-	Mus musculus
1.5.1.3	trimethoprim	folate antagonist drug	Mus musculus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.1.3	0.0022	-	NADPH	altered enzyme	Mus musculus
1.5.1.3	0.003	-	7,8-dihydrofolate	wild-type enzyme	Mus musculus
1.5.1.3	0.01	-	7,8-dihydrofolate	altered enzyme	Mus musculus
1.5.1.3	0.03	-	NADPH	wild-type	Mus musculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Mus musculus	-	altered enzyme encoded by amplified genes in cultured fibroblasts	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.5.1.3	fibroblast	cultured 3T6 mouse embryo fibroblasts	Mus musculus	-

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.5.1.3	additional information	-	1 mM NADPH protects against inactivation	Mus musculus
1.5.1.3	55	-	half-life: 8 min	Mus musculus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.5.1.3	0.243	-	7,8-dihydrofolate	altered enzyme	Mus musculus
1.5.1.3	4.67	-	7,8-dihydrofolate	wild-type enzyme	Mus musculus

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Release 2023.1 (January 2023)