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Literature summary extracted from

  • Gupta, R.S.; Flintoff, W.F.; Siminovitch, L.
    Purification and properties of dihydrofolate reductase from methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells (1977), Can. J. Biochem., 55, 445-452.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 24000
-
 ovary cells, SDS-PAGE Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Cricetulus griseus
-
 chinese hamster, methotrexate-sensitive and methotrexate-resistant cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 methotrexate-sensitive and methotrexate resistant cells Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 0.75
-
 methotrexate-sensitive strain Cricetulus griseus
1.5.1.3 0.85
-
 methotrexate-resistant strain Cricetulus griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 53
-
 wild-type: decay rate constant 0.12 per min, mutant enzymes 0.06-0.08 per min Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.5 5
-
 Cricetulus griseus