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Literature summary extracted from

  • Meek, T.D.; Garvey, E.P.; Santi, D.V.
    Purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant Leishmania tropica (1985), Biochemistry, 24, 678-686.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 5-methyltetrahydrofolate
-
 Leishmania tropica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Leishmania tropica
1.5.1.3 0.0015
-
 7,8-dihydrofolate
-
 Leishmania tropica
1.5.1.3 0.0027
-
 NADPH
-
 Leishmania tropica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 56400
-
 2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE Leishmania tropica
1.5.1.3 110000
-
 methotrexate resistant, bifunctional, glycerol density gradient centrifugation Leishmania tropica

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Leishmania tropica
-
 methotrexate resistant bifunctional enzyme: thymidylate synthase-dihydrofolate reductase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Leishmania tropica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
 Leishmania tropica

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 -20°C, 40% glycerol, pH 7.4, 5 mM dithiothreitol, 1 mM EDTA, 1 month Leishmania tropica
1.5.1.3 4°C, 40-50% loss of activity after 1 month Leishmania tropica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Leishmania tropica 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 dimer 2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE Leishmania tropica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 25
-
 assay at Leishmania tropica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.15
-
 5-methyltetrahydrofolate
-
 Leishmania tropica