Literature summary extracted from

Baccanari, D.P.; Tansik, R.L.; Paterson, S.J.; Stone, D.
Characterization and amino acid sequence of Neisseria gonorrhoeae dihydrofolate reductase (1984), J. Biol. Chem., 259, 12291-12298.

Application

EC Number	Application	Comment	Organism
1.5.1.3	medicine	enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer	Neisseria gonorrhoeae

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.5.1.3	methotrexate	-	Neisseria gonorrhoeae
1.5.1.3	pyrimethamine	-	Neisseria gonorrhoeae
1.5.1.3	trimethoprim	-	Neisseria gonorrhoeae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.1.3	0.002	-	7,8-dihydrofolate	-	Neisseria gonorrhoeae
1.5.1.3	0.01	-	NADPH	-	Neisseria gonorrhoeae

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.5.1.3	17300	18300	both forms, nondenaturing PAGE	Neisseria gonorrhoeae
1.5.1.3	18000	-	SDS-PAGE	Neisseria gonorrhoeae
1.5.1.3	173000	-	1 * 173000, both forms, SDS-PAGE	Neisseria gonorrhoeae

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.3	Neisseria gonorrhoeae	-	2 forms, not genetically determined isozymes	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.1.3	-	Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.1.3	7,8-dihydrofolate + NADPH + H+	-	Neisseria gonorrhoeae	5,6,7,8-tetrahydrofolate + NADP+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.5.1.3	monomer	1 * 173000, both forms, SDS-PAGE	Neisseria gonorrhoeae

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.5.1.3	additional information	-	additional information	-	Neisseria gonorrhoeae

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.1.3	NADPH	-	Neisseria gonorrhoeae

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.5.1.3	0.00002	-	trimethoprim	-	Neisseria gonorrhoeae
1.5.1.3	0.0003	-	pyrimethamine	-	Neisseria gonorrhoeae

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Release 2023.1 (January 2023)