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Literature summary extracted from

  • Erickson, J.S.; Mathews, C.K.
    Dihydrofolate reductases of Escherichia coli and bacteriophage Tr. A spectrofluorometric study (1973), Biochemistry, 12, 372-380.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 aminopterin
-
 Escherichia coli
1.5.1.3 aminopterin
-
 Tequatrovirus T4
1.5.1.3 Cl-
-
 Escherichia coli
1.5.1.3 Cl-
-
 Tequatrovirus T4
1.5.1.3 ClO4-
-
 Escherichia coli
1.5.1.3 ClO4-
-
 Tequatrovirus T4
1.5.1.3 F-
-
 Escherichia coli
1.5.1.3 F-
-
 Tequatrovirus T4
1.5.1.3 methotrexate
-
 Escherichia coli
1.5.1.3 methotrexate
-
 Tequatrovirus T4
1.5.1.3 N10-formylaminopterin competitive against 7,8-dihydrofolate and noncompetitive against NADPH Tequatrovirus T4
1.5.1.3 SCN-
-
 Escherichia coli
1.5.1.3 SCN-
-
 Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Escherichia coli
1.5.1.3 additional information
-
 additional information
-
 Tequatrovirus T4
1.5.1.3 0.0023
-
 7,8-dihydrofolate
-
 Tequatrovirus T4
1.5.1.3 0.018
-
 NADPH
-
 Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 22500
-
 B, SDS-PAGE Escherichia coli
1.5.1.3 29000
-
 SDS-PAGE Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-
1.5.1.3 Escherichia coli MB 1428 / B / ATCC 11303
-
-
-
1.5.1.3 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Escherichia coli
1.5.1.3
-
 Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
 Escherichia coli
1.5.1.3 additional information
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Tequatrovirus T4 5,6,7,8-tetrahydrofolate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 29000, SDS-PAGE Tequatrovirus T4
1.5.1.3 monomer 1 * 22500, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli
1.5.1.3 NADPH A-side specific Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.00018
-
 N10-formylaminopterin
-
 Tequatrovirus T4