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Literature summary extracted from

  • Poe, M.; Breeze, A.S.; Wu, J.K.; Short, C.R.; Hoogsteen, K.
    Dihydrofolate reductase from trimethoprim-resistant Escherichia coli MB 3746 and MB 3747. Purification, amino acid composition, and some kinetic properties (1979), J. Biol. Chem., 254, 1799-1805.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.3 7,8-dihydrofolate and folate protect against ethoxyformic anhydride modification Escherichia coli
1.5.1.3 7,8-dihydrofolate protects against heat inactivation Escherichia coli
1.5.1.3 7,8-dihydrofolate protects against inactivation Escherichia coli
1.5.1.3 NADPH, protects against ethoxyformic anhydride modification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 2,4-diaminopyrimidine
-
 Escherichia coli
1.5.1.3 aminopterin
-
 Escherichia coli
1.5.1.3 folate
-
 Escherichia coli
1.5.1.3 methotrexate
-
 Escherichia coli
1.5.1.3 trimethoprim
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 17300 18300 trimethoprim-resistant Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
 strains: MB3746, MB 3747 of K12
-
1.5.1.3 Escherichia coli
-
 trimethoprim-resistant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 strain MB 3746, MB 3747 Escherichia coli
1.5.1.3 trimethoprim-resistant Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 6.4
-
 below, strain 3747 Escherichia coli
1.5.1.3 7.6
-
 strain 1428 Escherichia coli
1.5.1.3 8
-
 strain 3746 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.000001
-
 trimethoprim strains MB 1428 and MB 3746 Escherichia coli
1.5.1.3 0.000011
-
 trimethoprim strain MB 3747 Escherichia coli