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Literature summary extracted from

  • Oe, H.; Kohashi, M.; Iwai, K.
    The occurence and properties of pteridine reductase: dihydrofolate reductase in Crithidia fasciculata (1983), Agric. Biol. Chem., 47, 251-258.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 chaotrophes e.g. guanidine-HCl; formamide; urea Crithidia fasciculata
1.5.1.3 folate
-
 Crithidia fasciculata
1.5.1.3 L-erythro-biopterin
-
 Crithidia fasciculata
1.5.1.3 methotrexate
-
 Crithidia fasciculata
1.5.1.3 N-ethylmaleimide
-
 Crithidia fasciculata
1.5.1.3 NADP+
-
 Crithidia fasciculata
1.5.1.3 p-chloromercuribenzoate
-
 Crithidia fasciculata
1.5.1.3 pyrimethamine
-
 Crithidia fasciculata
1.5.1.3 trimethoprim
-
 Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0009
-
 dihydropteroate
-
 Crithidia fasciculata
1.5.1.3 0.0021
-
 NADPH with dihydropteroate Crithidia fasciculata
1.5.1.3 0.0034
-
 6-Hydroxymethylpterin
-
 Crithidia fasciculata
1.5.1.3 0.0035
-
 L-threo-neopterin
-
 Crithidia fasciculata
1.5.1.3 0.0048
-
 7,8-dihydrofolate
-
 Crithidia fasciculata
1.5.1.3 0.0059
-
 NADPH with 7,8-dihydrofolate or 6-hydroxymetylpterin Crithidia fasciculata
1.5.1.3 0.011
-
 NADPH with L-threo-neopterin Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 110000
-
 gel filtration Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Crithidia fasciculata
-
 bifunctional enzyme: pteridine reductase-dihydrofolate reductase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 bifunctional enzyme: pteridine reductase-dihydrofolate reductase Crithidia fasciculata

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 ammonium sulfate, 55% saturation, 0°C, for at least 1 year Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 6-hydroxymethylpterin + NADPH
-
 Crithidia fasciculata 6-hydroxymethyl-7,8-dihydropterin + NADP+
-
 ?
1.5.1.3 6-methylpterin + NADPH
-
 Crithidia fasciculata 6-methyl-7,8-dihydropterin + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Crithidia fasciculata 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 D-erythro-7,8-neopterin + NADPH low activity Crithidia fasciculata ?
-
 ?
1.5.1.3 D-threo-neopterin + NADPH low activity Crithidia fasciculata ?
-
 ?
1.5.1.3 dihydropteroate + NADPH
-
 Crithidia fasciculata ?
-
 ?
1.5.1.3 folate + NADPH no activity Crithidia fasciculata 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 L-erythro-neopterin + NADPH
-
 Crithidia fasciculata ?
-
 ?
1.5.1.3 L-threo-7,8-dihydroneopterin + NADPH low activity Crithidia fasciculata ?
-
 ?
1.5.1.3 L-threo-neopterin + NADPH
-
 Crithidia fasciculata ?
-
 ?
1.5.1.3 additional information no activity with folate, sepiapterin, isoxanthopterin, leucopterin Crithidia fasciculata ?
-
 ?
1.5.1.3 pterin + NADPH low activity Crithidia fasciculata 7,8-dihydropterin + NADP+
-
 ?
1.5.1.3 xanthopterin + NADPH low activity Crithidia fasciculata ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 6.8
-
-
 Crithidia fasciculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADH not Crithidia fasciculata
1.5.1.3 NADPH
-
 Crithidia fasciculata

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.00034
-
 L-erythro-biopterin with 7,8-dihydrofolate Crithidia fasciculata
1.5.1.3 0.00072
-
 pyrimethamine with 7,8-dihydrofolate Crithidia fasciculata
1.5.1.3 0.0011
-
 methotrexate with 7,8-dihydrofolate Crithidia fasciculata
1.5.1.3 0.0064
-
 trimethoprim with 7,8-dihydrofolate Crithidia fasciculata
1.5.1.3 0.021
-
 NADP+ with 7,8-dihydrofolate Crithidia fasciculata
1.5.1.3 0.036
-
 folate with 7,8-dihydrofolate Crithidia fasciculata