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Literature summary extracted from

  • Fontecave, M.; Eliasson, R.; Reichard, P.
    NAD(P)H:flavin oxidoreductase of Escherichia coli (1987), J. Biol. Chem., 262, 12325-12331.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.41 dilute solutions can be stabilized by the addition of bovine serum albumin (0.1 mg/ml) and/or by glycerol Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.41 iodoacetate
-
 Escherichia coli
1.5.1.41 additional information no inhibition by EDTA, desferal or dicoumarol Escherichia coli
1.5.1.41 N-ethylmaleimide
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.41 0.0006
-
 riboflavin second substrate: NADH, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.0008
-
 FAD second substrate: NADH, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.0008
-
 FMN second substrate: NADH, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.0016
-
 riboflavin second substrate: NADPH, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.002
-
 FMN second substrate: NADPH, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.025
-
 NADH second substrate riboflavin, pH 7.5, 23°C Escherichia coli
1.5.1.41 0.043
-
 NADPH second substrate riboflavin, pH 7.5, 23°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.41 28000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.41 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.41
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.41 0.36
-
 pH 7.5, 23°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.41 FAD + NADH + H+ no activity with FAD + NADPH Escherichia coli FADH2 + NAD+
-
 ?
1.5.1.41 FMN + NADH + H+
-
 Escherichia coli FMNH2 + NAD+
-
 ?
1.5.1.41 FMN + NADPH + H+ very low activity Escherichia coli FMNH2 + NADP+
-
 ?
1.5.1.41 riboflavin + NADH + H+
-
 Escherichia coli reduced riboflavin + NAD+
-
 ?
1.5.1.41 riboflavin + NADPH + H+
-
 Escherichia coli reduced riboflavin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.41 monomer 1 * 28000-29000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.5.1.41 NAD(P)H:flavin oxidoreductase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.41 23
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.41 7.5
-
 assay at Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.41 7.4
-
 stable at pH 7.4, except at low protein concentrations Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.41 Escherichia coli isoelectric focusing
-
 4.9