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Literature summary extracted from

  • Sackett, D.L.; Ruvinov, S.B.; Thompson, J.
    N5-(L-1-carboxyethyl)-L-ornithine synthase: Physical and spectral characterization of teh enzyme and its unusual low pKa fluorescent tyrosine residues (1999), Protein Sci., 8, 2121-2129.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.5.1.24 nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.24 ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571 Lactococcus lactis
1.5.1.24 gene ceo, encoding the tetrameric enzyme, cloned and sequenced, plasmid p493 containing ceo transformed into Escherichia coli TG1 Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.24
-
 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.24 35320
-
 deduced from amino acid sequence Lactococcus lactis
1.5.1.24 37000
-
 4 * 37000, SDS-PAGE Lactococcus lactis
1.5.1.24 137000
-
 XLA Lactococcus lactis
1.5.1.24 140000
-
 native PAGE Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.24 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.24 recombinant enzyme purified from the overproducing strain Escherichia coli CEO571 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.24 L-ornithine + pyruvate + NADPH
-
 Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.5.1.24 tetramer 4 * 37000, SDS-PAGE Lactococcus lactis
1.5.1.24 tetramer 4* 35355, MALDI-MS Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.24 CEOS
-
 Lactococcus lactis