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Literature summary extracted from

  • Donkersloot, J.A.; Thompson, J.
    Cloning, expression, sequence analysis, and site-directed mutagenesis of the Tn5306-encoded N5-(carboxyethyl)ornithine synthase from Lactococcus lactis K1 (1995), J. Biol. Chem., 270, 12226-12234.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.1.24 nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis
1.5.1.24 synthesis enzymatic biosyntheses of amino acids instead of chemical syntheses provide an attractive alternative to the former procedures since they are efficient and simple to perform. N,N-dialkylation does not occur and the enzyme-catalyzed reactions are both regio-and stereochemically specific Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.24 ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.24 35320
-
 deduced from amino acid sequence Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.24 Lactococcus lactis
-
 subsp. lactis K1, formerly Streptococcus lactis K1
-
1.5.1.24 Lactococcus lactis
-
 formerly Streptococcus lactis K1-23
-
1.5.1.24 Lactococcus lactis K1-23
-
 formerly Streptococcus lactis K1-23
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.24
-
 Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.24 L-ornithine + pyruvate + NADPH
-
 Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
1.5.1.24 L-ornithine + pyruvate + NADPH
-
 Lactococcus lactis K1-23 N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
1.5.1.24 lysine + pyruvate + NADPH
-
 Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir
1.5.1.24 lysine + pyruvate + NADPH
-
 Lactococcus lactis K1-23 N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.5.1.24 N5-(CE) ornithine synthase
-
 Lactococcus lactis