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Literature summary extracted from

  • Thompson, J.
    N5-(L-1-carboxyethyl)-L-ornithine:NADP+ oxidoreductase from Streptococcus lactis. Purification and partial characterization (1989), J. Biol. Chem., 264, 9592-9601.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.24 addition of 20% glycerol stabilizes the enzyme Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.24 N5-(L-1-carboxyethyl)-L-ornithine
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.24 0.0066
-
 NADPH
-
 Lactococcus lactis
1.5.1.24 0.15
-
 pyruvate
-
 Lactococcus lactis
1.5.1.24 3.3
-
 ornithine
-
 Lactococcus lactis
1.5.1.24 18.2
-
 lysine
-
 Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.24 37000 38000 immunoblot analysis Lactococcus lactis
1.5.1.24 38000
-
 2 * 38000, SDS-PAGE Lactococcus lactis
1.5.1.24 38000
-
 4 * 38000, SDS-PAGE Lactococcus lactis
1.5.1.24 39200
-
 calculated from amino acid sequence Lactococcus lactis
1.5.1.24 78000
-
 gel filtration Lactococcus lactis
1.5.1.24 150000
-
 PAGE, hight pressure liquid chromatography, gel filtration Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.24 Lactococcus lactis
-
 subsp. lactis K1, formerly Streptococcus lactis K1
-
1.5.1.24 Lactococcus lactis
-
 strains S.lactis K1 and 133
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.24
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.24 40.5
-
-
 Lactococcus lactis

Storage Stability

EC Number Storage Stability Organism
1.5.1.24 -20°C, no significant loss of activity detected after 2 months of storage Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.24 L-ornithine + pyruvate + NADPH pyruvic acid serves as an effective keto substrate for the condensation reaction Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ir
1.5.1.24 lysine + pyruvate + NADPH
-
 Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
 ir
1.5.1.24 ornithine + 3-fluoropyruvate + NADPH slight activity observed, about 15% of the rate found with pyruvate Lactococcus lactis N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.24 dimer 2 * 38000, SDS-PAGE Lactococcus lactis
1.5.1.24 tetramer 4 * 38000, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.24 N5-CEO synthase
-
 Lactococcus lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.1.24 25 50
-
 Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.24 50
-
 stable to heating for 10 min Lactococcus lactis
1.5.1.24 60
-
 rapidly inactivated by heating at Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.24 8
-
-
 Lactococcus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.24 6.5 9
-
 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.24 NADPH high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor Lactococcus lactis