EC Number | General Stability | Organism |
---|---|---|
1.5.1.24 | addition of 20% glycerol stabilizes the enzyme | Lactococcus lactis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.24 | N5-(L-1-carboxyethyl)-L-ornithine | - |
Lactococcus lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.24 | 0.0066 | - |
NADPH | - |
Lactococcus lactis | |
1.5.1.24 | 0.15 | - |
pyruvate | - |
Lactococcus lactis | |
1.5.1.24 | 3.3 | - |
ornithine | - |
Lactococcus lactis | |
1.5.1.24 | 18.2 | - |
lysine | - |
Lactococcus lactis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 37000 | 38000 | immunoblot analysis | Lactococcus lactis |
1.5.1.24 | 38000 | - |
2 * 38000, SDS-PAGE | Lactococcus lactis |
1.5.1.24 | 38000 | - |
4 * 38000, SDS-PAGE | Lactococcus lactis |
1.5.1.24 | 39200 | - |
calculated from amino acid sequence | Lactococcus lactis |
1.5.1.24 | 78000 | - |
gel filtration | Lactococcus lactis |
1.5.1.24 | 150000 | - |
PAGE, hight pressure liquid chromatography, gel filtration | Lactococcus lactis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.24 | Lactococcus lactis | - |
subsp. lactis K1, formerly Streptococcus lactis K1 | - |
1.5.1.24 | Lactococcus lactis | - |
strains S.lactis K1 and 133 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.24 | - |
Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 40.5 | - |
- |
Lactococcus lactis |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.24 | -20°C, no significant loss of activity detected after 2 months of storage | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.24 | L-ornithine + pyruvate + NADPH | pyruvic acid serves as an effective keto substrate for the condensation reaction | Lactococcus lactis | N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O | - |
ir | |
1.5.1.24 | lysine + pyruvate + NADPH | - |
Lactococcus lactis | N7-(1-carboxyethyl)lysine + NADP+ + H2O | - |
ir | |
1.5.1.24 | ornithine + 3-fluoropyruvate + NADPH | slight activity observed, about 15% of the rate found with pyruvate | Lactococcus lactis | N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.24 | dimer | 2 * 38000, SDS-PAGE | Lactococcus lactis |
1.5.1.24 | tetramer | 4 * 38000, SDS-PAGE | Lactococcus lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.24 | N5-CEO synthase | - |
Lactococcus lactis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 25 | 50 | - |
Lactococcus lactis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 50 | - |
stable to heating for 10 min | Lactococcus lactis |
1.5.1.24 | 60 | - |
rapidly inactivated by heating at | Lactococcus lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 8 | - |
- |
Lactococcus lactis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.24 | 6.5 | 9 | - |
Lactococcus lactis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.24 | NADPH | high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor | Lactococcus lactis |