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Literature summary extracted from

  • Zhou, J.; Kang, S.S.; Wong, P.W.K.; Fournier, B.; Rozen, R.
    Purification and characterization of methylenetetrahydrofolate reductase from human cadaver liver [published erratum appears in Biochem Med Metab Biol 1990 Oct;44(2):200] (1990), Biochem. Med. Metab. Biol., 43, 234-242.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.20 extremely sensitive to proteolysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 S-adenosylmethionine inhibition of the enzyme in crude extracts from fresh liver biopsies, but not after purification from cadaver liver Homo sapiens
1.5.1.20 tetrahydrofolate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.20 0.012
-
 5,10-methylenetetrahydrofolate NADPH-5,10-methylenetetrahydrofolate-oxidoreductase reaction Homo sapiens
1.5.1.20 0.017
-
 NADPH NADPH-menadione oxidoreductase reaction Homo sapiens
1.5.1.20 0.038
-
 5-methyltetrahydrofolate 5,10-methylenetetrahydrofolate-oxidoreductase reaction Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.20 75000
-
 SDS-PAGE Homo sapiens
1.5.1.20 150000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH Homo sapiens first step in biosynthesis of methyl groups ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.20 homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.20 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.20 0.367
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH
-
 Homo sapiens 5-methyltetrahydrofolate + NADP+
-
 ?
1.5.1.20 5,10-methylenetetrahydrofolate + NADPH first step in biosynthesis of methyl groups Homo sapiens ?
-
 ?
1.5.1.20 5-methyltetrahydrofolate + oxidized menadione
-
 Homo sapiens 5,10-methylenetetrahydrofolate + reduced menadione
-
 ?
1.5.1.20 NADPH + H+ + menadione
-
 Homo sapiens NADP+ + menadiol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.20 dimer 2 * 75000, SDS-PAGE and inhibition studies Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.20 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.20 6.5
-
 NADPH-tetrahydrofolate oxidoreductase activity Homo sapiens
1.5.1.20 6.6
-
 methyltetrahydrofolate-menadione oxidoreductase activity Homo sapiens
1.5.1.20 7.2
-
 NADPH-menadione oxidoreductase activity Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 FAD required Homo sapiens