EC Number | General Stability | Organism |
---|---|---|
1.5.1.53 | inclusion of 10% glycerol during purification is essential for stability | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.53 | 0.016 | - |
NADPH | - |
Sus scrofa | |
1.5.1.53 | 0.019 | - |
5,10-methylenetetrahydrofolate | - |
Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.53 | 74500 | - |
calculated from amino acid sequence | Sus scrofa |
1.5.1.53 | 77300 | - |
SDS-PAGE | Sus scrofa |
1.5.1.53 | 210000 | - |
gel filtration | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.53 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.53 | homogeneity | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.53 | liver | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.53 | 19.4 | - |
- |
Sus scrofa |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.53 | -20°C, for at least 2 weeks in 10% glycerol | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.53 | 5,10-methylenetetrahydrofolate + NADPH | - |
Sus scrofa | 5-methyltetrahydrofolate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.53 | dimer | 2 * 77300, SDS-PAGE, gel filtration | Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.53 | 37 | - |
assay at | Sus scrofa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.53 | 26.7 | - |
NADPH | - |
Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.53 | FAD | flavoprotein | Sus scrofa | |
1.5.1.53 | FAD | each subunit of the dimer or trimer contains bound FAD | Sus scrofa |