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Literature summary extracted from

  • Kenklies, J.; Ziehn, R.; Fritsche, K.; Pich, A.; Andreesen, J.R.
    Proline biosynthesis from L-ornithine in Clostridium sticklandii: purification of DELTA1-pyrroline-5-carboxylate reductase, and sequence and expression of the encoding gene, proC (1999), Microbiology, 145, 819-826.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2
-
Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 ATP 70% inhibition at 2 mM Acetoanaerobium sticklandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.025
-
NADH
-
Acetoanaerobium sticklandii
1.5.1.2 0.19
-
1-pyrroline-5-carboxylate
-
Acetoanaerobium sticklandii
1.5.1.2 1.55
-
L-proline L-proline oxidation Acetoanaerobium sticklandii
1.5.1.2 10.5
-
NAD+ L-proline oxidation Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.2 Mg2+
-
Acetoanaerobium sticklandii
1.5.1.2 potassium acetate increase at 3 mM Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 25000
-
8 * 25000 SDS-PAGE Acetoanaerobium sticklandii
1.5.1.2 230000
-
gel filtration Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 homogeneity Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 173.4
-
-
Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ reversible at pH values above 8 and higher substrate concentrations, oxidation of L-proline is strictly specific for the L-isomer Acetoanaerobium sticklandii L-proline + NAD(P)+
-
r
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
Acetoanaerobium sticklandii L-proline + NAD+
-
r
1.5.1.2 L-proline + NAD+
-
Acetoanaerobium sticklandii 1-pyrroline-5-carboxylate + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.5.1.2 octamer 8 * 25000 SDS-PAGE Acetoanaerobium sticklandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 7.4
-
-
Acetoanaerobium sticklandii
1.5.1.2 10
-
L-proline oxidation Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH preferentially Acetoanaerobium sticklandii
1.5.1.2 NADH L-proline oxidation is strictly NAD+ dependent Acetoanaerobium sticklandii
1.5.1.2 NADPH
-
Acetoanaerobium sticklandii