EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.2 | additional information | - |
up to 480000 kDa, quarternary structure changes drastically with buffer environment | Hordeum vulgare |
1.5.1.2 | 30000 | - |
- |
Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.2 | Hordeum vulgare | - |
barley | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.2 | homogeneity | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.2 | seedling | - |
Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.2 | 507 | - |
- |
Hordeum vulgare |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.2 | -80°C, 5% glycerol, 2 mM dithiothreitol, 1 month | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.2 | 1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Hordeum vulgare | L-proline + NAD(P)+ | - |
? | |
1.5.1.2 | L-proline benzyl ester + NAD+ | 24% of activity with proline | Hordeum vulgare | L-pyrroline-5-carboxy benzyl ester + NADH | - |
? | |
1.5.1.2 | L-proline methyl ester + NAD+ | 16% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy methyl ester + NADH | - |
? | |
1.5.1.2 | L-proline t-butyl ester + NAD+ | 10% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy tert-butylester + NADH | - |
? | |
1.5.1.2 | additional information | not: D-proline, L-hydroxyproline, L-azatidine-2-carboxylate, no other amino acids | Hordeum vulgare | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.2 | polymer | 12-16 * 30000 SDS-PAGE | Hordeum vulgare |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.2 | 1270 | - |
pyrroline-5-carboxylate | - |
Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.2 | 6.8 | 7.5 | pyrroline-5-carboxylate reductase activity | Hordeum vulgare |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.2 | 8.5 | - |
no proline dehydrogenase activity below pH 8.5 | Hordeum vulgare |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.2 | NADH | - |
Hordeum vulgare | |
1.5.1.2 | NADPH | - |
Hordeum vulgare |