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Literature summary extracted from

  • Krueger, R.; Jäger, H.J.; Hintz, M.; Pahlich, E.
    Purification to homogeneity of pyrroline-5-carboxylate reductase of barley (1986), Plant Physiol., 80, 142-144.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 additional information
-
up to 480000 kDa, quarternary structure changes drastically with buffer environment Hordeum vulgare
1.5.1.2 30000
-
-
Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Hordeum vulgare
-
barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 homogeneity Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 seedling
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 507
-
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 -80°C, 5% glycerol, 2 mM dithiothreitol, 1 month Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+
-
Hordeum vulgare L-proline + NAD(P)+
-
?
1.5.1.2 L-proline benzyl ester + NAD+ 24% of activity with proline Hordeum vulgare L-pyrroline-5-carboxy benzyl ester + NADH
-
?
1.5.1.2 L-proline methyl ester + NAD+ 16% of activity with proline Hordeum vulgare 1-pyrroline-5-carboxy methyl ester + NADH
-
?
1.5.1.2 L-proline t-butyl ester + NAD+ 10% of activity with proline Hordeum vulgare 1-pyrroline-5-carboxy tert-butylester + NADH
-
?
1.5.1.2 additional information not: D-proline, L-hydroxyproline, L-azatidine-2-carboxylate, no other amino acids Hordeum vulgare ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 polymer 12-16 * 30000 SDS-PAGE Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 1270
-
pyrroline-5-carboxylate
-
Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.8 7.5 pyrroline-5-carboxylate reductase activity Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.2 8.5
-
no proline dehydrogenase activity below pH 8.5 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
Hordeum vulgare
1.5.1.2 NADPH
-
Hordeum vulgare