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Literature summary extracted from

  • Hagedorn, C.H.
    Demonstration of a NADPH-linked delta 1-pyrroline-5-carboxylate-proline shuttle in a cell-free rat liver system (1986), Biochim. Biophys. Acta, 884, 11-17.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.024 0.025 1-pyrroline-5-carboxylate
-
 Rattus norvegicus
1.5.1.2 0.62
-
 DL-pyrroline-5-carboxylate cosubstrate NADH Rattus norvegicus
1.5.1.2 1.62 1.64 1-pyrroline-5-carboxylate
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Rattus norvegicus
-
 male Sprague-Dawley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 -70°C, 50 mM triethanolamine buffer pH 7.4, 9% w/v sucrose, 1 mM EDTA, 2 mM MgCl2, 30 mM 2-mercaptoethanol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NAD(P)H + H+ transfers the proS hydrogen at C-4 of the dihydropyridine ring of NAD(P)H to its substrate Rattus norvegicus L-proline + NAD(P)+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Rattus norvegicus L-proline + NAD+
-
 r
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Rattus norvegicus L-proline + NADP+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2
-
-
 30 min, loss of activity 16%-99%, highly dependent on buffer Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADPH preferentially Rattus norvegicus