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Literature summary extracted from

  • Appling, D.R.; West, M.G.
    Monofunctional NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae (1997), Methods Enzymol., 281, 178-188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.15 yeast gene MTD1 cloned and sequenced Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.15 cytoplasm
-
 Drosophila melanogaster 5737
-
1.5.1.15 cytoplasm
-
 Mus musculus 5737
-
1.5.1.15 cytoplasm
-
 Homo sapiens 5737
-
1.5.1.15 cytoplasm
-
 Saccharomyces cerevisiae 5737
-
1.5.1.15 mitochondrion
-
 Drosophila melanogaster 5739
-
1.5.1.15 mitochondrion
-
 Mus musculus 5739
-
1.5.1.15 mitochondrion
-
 Homo sapiens 5739
-
1.5.1.15 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 33000 38000
-
 Saccharomyces cerevisiae
1.5.1.15 36125
-
 2 * 36125, electrospray ionization-mass spectrometry Saccharomyces cerevisiae
1.5.1.15 64000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Drosophila melanogaster
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Drosophila melanogaster
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Acetobacterium woodii
-
-
-
1.5.1.15 Clostridium formicaceticum
-
-
-
1.5.1.15 Drosophila melanogaster
-
-
-
1.5.1.15 Homo sapiens
-
-
-
1.5.1.15 Mus musculus
-
-
-
1.5.1.15 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15 native enzyme from yeast as well as recombinant yest enzyme expressed in Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 5.38
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Drosophila melanogaster L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Drosophila melanogaster 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 additional information
-
 Mus musculus ?
-
 ?
1.5.1.15 additional information
-
 Homo sapiens ?
-
 ?
1.5.1.15 additional information
-
 Clostridium formicaceticum ?
-
 ?
1.5.1.15 additional information contains 2 enzymes, monofunctional NAD+ dependent dehydrogenase EC1.5.1.15 and trifunctional NADP+ dependent dehydrogenase, EC1.5.1.5 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.15 dimer
-
 Mus musculus
1.5.1.15 dimer
-
 Clostridium formicaceticum
1.5.1.15 dimer
-
 Acetobacterium woodii
1.5.1.15 dimer 2 * 36125, electrospray ionization-mass spectrometry Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
 Mus musculus
1.5.1.15 NAD+
-
 Homo sapiens
1.5.1.15 NAD+
-
 Saccharomyces cerevisiae
1.5.1.15 NAD+
-
 Clostridium formicaceticum
1.5.1.15 NAD+
-
 Acetobacterium woodii