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Literature summary extracted from

  • Yang, X.M.; Mackenzie, R.E.
    NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene (1993), Biochemistry, 32, 11118-11123.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.15 phosphate
-
 Mus musculus
1.5.1.15 phosphate absolute requirement, phosphate can be replaced by its analog arsenate Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.15 recombinant enzyme, expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.0046
-
 L-5,10-methylenetetrahydrofolate
-
 Homo sapiens
1.5.1.15 0.18
-
 Mg2+
-
 Homo sapiens
1.5.1.15 2.5
-
 NAD+
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.15 cytosol
-
 Mus musculus 5829
-
1.5.1.15 cytosol
-
 Homo sapiens 5829
-
1.5.1.15 cytosol
-
 Saccharomyces cerevisiae 5829
-
1.5.1.15 mitochondrion
-
 Mus musculus 5739
-
1.5.1.15 mitochondrion
-
 Homo sapiens 5739
-
1.5.1.15 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Mg2+
-
 Mus musculus
1.5.1.15 Mg2+ absolute requirement Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Homo sapiens
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Homo sapiens
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Homo sapiens
-
 human
-
1.5.1.15 Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
1.5.1.15 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15 from ascites tumor cells Mus musculus
1.5.1.15 recombinant enzyme, expressed in Escherichia coli Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.15 Ehrlich ascites carcinoma cell
-
 Mus musculus
-
1.5.1.15 fetus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Homo sapiens L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Homo sapiens 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 additional information
-
 Mus musculus ?
-
 ?
1.5.1.15 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
1.5.1.15 additional information bifunctional enzyme Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.15 Mg2+-/phosphate-dependent dehydrogenase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
 Mus musculus
1.5.1.15 NAD+
-
 Saccharomyces cerevisiae
1.5.1.15 NAD+ NAD+ cofactor can be replaced by NADP+ Homo sapiens