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Literature summary extracted from

  • Moore, M.R.; O'Brien, W.E.; Ljungdahl, L.G.
    Purification and characterisation of nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum (1974), J. Biol. Chem., 249, 5250-5253.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.15 EDTA NAD+ dependent enzyme from Ehrlich ascites tumor cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.066
-
 L-5,10-methylenetetrahydrofolate
-
 Clostridium formicaceticum
1.5.1.15 0.79
-
 NAD+
-
 Clostridium formicaceticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Mg2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus
1.5.1.15 Mn2+ NAD+ dependent enzyme from Ehrlich ascites tumor cells requires Mg2+ or Mn2+ for activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 32000
-
 SDS-PAGE Clostridium formicaceticum
1.5.1.15 60000
-
 sedimentation equilibrium centrifugation Clostridium formicaceticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Saccharomyces cerevisiae
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Gottschalkia acidurici
-
 L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Saccharomyces cerevisiae
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Gottschalkia acidurici
-
 5,10-methenyl-tetrahydrofolate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Clostridium formicaceticum
-
 strain ATCC 23439
-
1.5.1.15 Gottschalkia acidurici
-
-
-
1.5.1.15 Mus musculus
-
 mouse, Ehrlich ascites tumor cells
-
1.5.1.15 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15
-
 Saccharomyces cerevisiae
1.5.1.15
-
 Clostridium formicaceticum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.15 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 1400
-
-
 Clostridium formicaceticum

Storage Stability

EC Number Storage Stability Organism
1.5.1.15 4°C, can be stored dissolved in 0.1 M potassium maleate, pH 7.0 for at least 1 month without significant loss of activity Clostridium formicaceticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Saccharomyces cerevisiae L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
 Gottschalkia acidurici L-5,10-methylenetetrahydrofolate + NAD+
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Saccharomyces cerevisiae 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
 Gottschalkia acidurici 5,10-methenyl-tetrahydrofolate + NADH
-
 r
1.5.1.15 additional information
-
 Mus musculus ?
-
 ?
1.5.1.15 additional information
-
 Saccharomyces cerevisiae ?
-
 ?
1.5.1.15 additional information active only with L-5,10-methylenetetrahydrofolate, the L-isomer does not affect the reaction Clostridium formicaceticum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.15 dimer
-
 Mus musculus
1.5.1.15 dimer
-
 Saccharomyces cerevisiae
1.5.1.15 dimer 2 * 32000, SDS-PAGE Clostridium formicaceticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.15 45
-
 activity increases to the optimum, above 45°C the enzyme begins to denature Clostridium formicaceticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.15 50
-
 stable for 30 min at temperatures below, loses 25% of its activity during incubation Clostridium formicaceticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
 Saccharomyces cerevisiae
1.5.1.15 NAD+ active with both NAD+ and NADP+ Gottschalkia acidurici
1.5.1.15 NAD+ specific for NAD+, no activity with NADP+ Clostridium formicaceticum
1.5.1.15 NAD+ Ehrlich ascites tumor cells contains 2 enzymes, NAD+ dependent and NADP+ dependent dehydrogenase Mus musculus
1.5.1.15 NADP+ active with both NAD+ and NADP+ Gottschalkia acidurici