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Literature summary extracted from

  • Schaap, P.J.; Muller, Y.; Sonnenberg, A.S.; van Griensven, L.J.; Visser, J.
    The Agaricus bisporus pruA gene encodes a cytosolic delta 1-pyrroline-5-carboxylate dehydrogenase which is expressed in fruit bodies but not in gill tissue (1997), Appl. Environ. Microbiol., 63, 57-62.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.88 agriculture possible target for upleveled nitrogen usage in commercial mushroom breeding Agaricus bisporus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.88 pruA gene, nucleotide sequence and deduced amino acid sequence thereof Agaricus bisporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.88 cytosol
-
 Agaricus bisporus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O Agaricus bisporus
-
 L-glutamate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Agaricus bisporus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.88 L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH + H+ no induction by ammonia Agaricus bisporus
a
1.2.1.88 L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH + H+ enzyme is a gamma-glutamyl semialdehyde dehydrogenase Agaricus bisporus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.88 fruit body not in mycelium or gill Agaricus bisporus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 0.0125
-
-
 Agaricus bisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Agaricus bisporus L-glutamate + NADH
-
 ?
1.2.1.88 additional information enzyme involved in L-proline catabolism Agaricus bisporus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.88 P5C dehydrogenase
-
 Agaricus bisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.88 NAD+
-
 Agaricus bisporus