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Literature summary extracted from

  • Isobe, K.; Matsuzawa, T.; Soda, K.
    Crystallization and characterization of 1-pyrroline-5-carboxylate dehydrogenase from Bacillus sphaericus (1987), Agric. Biol. Chem., 51, 1947-1953.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.88
-
 Lysinibacillus sphaericus

General Stability

EC Number General Stability Organism
1.2.1.88 NADP+ stabilizes Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 0.0095
-
 NADP+
-
 Lysinibacillus sphaericus
1.2.1.88 0.42
-
 1-pyrroline-5-carboxylate with NADP+ Lysinibacillus sphaericus
1.2.1.88 0.75
-
 1-pyrroline-5-carboxylate with NAD+ Lysinibacillus sphaericus
1.2.1.88 2.3
-
 NAD+
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 50000
-
 2 * 50000, SDS-PAGE Lysinibacillus sphaericus
1.2.1.88 100000
-
 gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Geobacillus stearothermophilus
-
 strains IFO 12983, ATCC 8005 and ATCC 21365
-
1.2.1.88 Geobacillus thermodenitrificans
-
 strains ATCC 39492 and DSM 465
-
1.2.1.88 Lysinibacillus sphaericus
-
-
-
1.2.1.88 [Bacillus] caldotenax
-
 strain DSM 406
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.88
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 2.6
-
 strain ATCC 21365 Geobacillus stearothermophilus
1.2.1.88 2.6
-
 strain ATCC 39492 Geobacillus thermodenitrificans
1.2.1.88 2.7
-
-
 [Bacillus] caldotenax
1.2.1.88 3.3
-
 strain DSM 465 Geobacillus thermodenitrificans
1.2.1.88 7.1
-
 strain ATCC 8005 Geobacillus stearothermophilus
1.2.1.88 8
-
 strain IFO 12983 Geobacillus stearothermophilus
1.2.1.88 34.1
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Geobacillus stearothermophilus L-glutamate + NADH
-
 ir
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 [Bacillus] caldotenax L-glutamate + NADH
-
 ir
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Geobacillus thermodenitrificans L-glutamate + NADH
-
 ir
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O highly specific Lysinibacillus sphaericus L-glutamate + NADH
-
 ir
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Lysinibacillus sphaericus L-glutamate + NADH + H+
-
 r
1.2.1.88 1-pyrroline-5-carboxylate + NADP+ + H2O
-
 Lysinibacillus sphaericus L-glutamate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.88 dimer 2 * 50000, SDS-PAGE Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.88 35
-
 cofactor NAD+ Lysinibacillus sphaericus
1.2.1.88 45
-
 cofactor NADP+ Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.88 35
-
 15 min, 40% loss of activity in presence of NAD+, 2% loss of activity in presence of NADP+ Lysinibacillus sphaericus
1.2.1.88 40
-
 15 min, 75% loss of activity in presence of NAD+, 10% loss of activity in presence of NADP+ Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.88 6.5 7
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.88 5.5 7.5 cofactor NAD+ Lysinibacillus sphaericus
1.2.1.88 5.5 8 cofactor NADP+ Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.88 NAD+
-
 Geobacillus stearothermophilus
1.2.1.88 NAD+
-
 Lysinibacillus sphaericus
1.2.1.88 NAD+
-
 [Bacillus] caldotenax
1.2.1.88 NAD+
-
 Geobacillus thermodenitrificans
1.2.1.88 NADP+ 8.1times higher activity compared to NAD+ Lysinibacillus sphaericus