BRENDA - Enzyme Database show

Occurrence of two forms of glutamate synthase in Chlamydomonas reinhardii

Cullimore, J.V.; Sims, A.P.; Phytochemistry 20, 597-600 (1981)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.4.1.14
2-mercaptoethanol stabilizes
Chlamydomonas reinhardtii
1.4.1.14
high levels of sucrose stabilize
Chlamydomonas reinhardtii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.14
azaserine
-
Chlamydomonas reinhardtii
1.4.7.1
azaserine
-
Chlamydomonas reinhardtii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.14
0.007
-
2-oxoglutarate
-
Chlamydomonas reinhardtii
1.4.1.14
0.013
-
NADH
-
Chlamydomonas reinhardtii
1.4.1.14
0.9
-
L-glutamine
-
Chlamydomonas reinhardtii
1.4.7.1
0.17
-
2-oxoglutarate
-
Chlamydomonas reinhardtii
1.4.7.1
0.19
-
L-glutamine
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.14
240000
-
sedimentation studies
Chlamydomonas reinhardtii
1.4.7.1
165000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
Chlamydomonas reinhardtii
ammonia assimilation
glutamate + NAD+
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
Chlamydomonas reinhardtii CCAP 11/32A
ammonia assimilation
glutamate + NAD+
-
-
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Chlamydomonas reinhardtii
-
L-glutamate + oxidized ferredoxin
-
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Chlamydomonas reinhardtii CCAP 11/32A
-
L-glutamate + oxidized ferredoxin
-
Chlamydomonas reinhardtii CCAP 11/32A
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.14
Chlamydomonas reinhardtii
-
-
-
1.4.1.14
Chlamydomonas reinhardtii CCAP 11/32A
-
-
-
1.4.7.1
Chlamydomonas reinhardtii
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.4.1.14
sensitive to oxygen
Chlamydomonas reinhardtii
Purification (Commentary)
EC Number
Commentary
Organism
1.4.1.14
partial purification
Chlamydomonas reinhardtii
1.4.7.1
partially purified
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
specific for NADH
392019
Chlamydomonas reinhardtii
L-glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
specific for NADH
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
ammonia assimilation
392019
Chlamydomonas reinhardtii
glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
ammonia assimilation
392019
Chlamydomonas reinhardtii CCAP 11/32A
glutamate + NAD+
-
-
-
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392019
Chlamydomonas reinhardtii
L-glutamate + oxidized ferredoxin
-
392019
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + oxidized ferredoxin
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392019
Chlamydomonas reinhardtii
L-glutamate + oxidized methylviologen
-
392019
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + oxidized methylviologen
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.1.14
30
-
assay at
Chlamydomonas reinhardtii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.1.14
additional information
-
cold-labile enzyme
Chlamydomonas reinhardtii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.14
7.5
-
-
Chlamydomonas reinhardtii
1.4.7.1
7.5
-
-
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.14
additional information
no activity with NADPH
Chlamydomonas reinhardtii
1.4.1.14
NADH
specific for NADH as reductant
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.14
additional information
no activity with NADPH
Chlamydomonas reinhardtii
1.4.1.14
NADH
specific for NADH as reductant
Chlamydomonas reinhardtii
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.1.14
2-mercaptoethanol stabilizes
Chlamydomonas reinhardtii
1.4.1.14
high levels of sucrose stabilize
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.14
azaserine
-
Chlamydomonas reinhardtii
1.4.7.1
azaserine
-
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.1.14
0.007
-
2-oxoglutarate
-
Chlamydomonas reinhardtii
1.4.1.14
0.013
-
NADH
-
Chlamydomonas reinhardtii
1.4.1.14
0.9
-
L-glutamine
-
Chlamydomonas reinhardtii
1.4.7.1
0.17
-
2-oxoglutarate
-
Chlamydomonas reinhardtii
1.4.7.1
0.19
-
L-glutamine
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.1.14
240000
-
sedimentation studies
Chlamydomonas reinhardtii
1.4.7.1
165000
-
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
Chlamydomonas reinhardtii
ammonia assimilation
glutamate + NAD+
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
Chlamydomonas reinhardtii CCAP 11/32A
ammonia assimilation
glutamate + NAD+
-
-
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Chlamydomonas reinhardtii
-
L-glutamate + oxidized ferredoxin
-
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
Chlamydomonas reinhardtii CCAP 11/32A
-
L-glutamate + oxidized ferredoxin
-
Chlamydomonas reinhardtii CCAP 11/32A
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.4.1.14
sensitive to oxygen
Chlamydomonas reinhardtii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.14
partial purification
Chlamydomonas reinhardtii
1.4.7.1
partially purified
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
specific for NADH
392019
Chlamydomonas reinhardtii
L-glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
specific for NADH
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
ammonia assimilation
392019
Chlamydomonas reinhardtii
glutamate + NAD+
-
-
-
?
1.4.1.14
L-glutamine + 2-oxoglutarate + NADH
ammonia assimilation
392019
Chlamydomonas reinhardtii CCAP 11/32A
glutamate + NAD+
-
-
-
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392019
Chlamydomonas reinhardtii
L-glutamate + oxidized ferredoxin
-
392019
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + oxidized ferredoxin
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392019
Chlamydomonas reinhardtii
L-glutamate + oxidized methylviologen
-
392019
Chlamydomonas reinhardtii
?
1.4.7.1
L-glutamine + 2-oxoglutarate + reduced methylviologen
artificial elctron donor
392019
Chlamydomonas reinhardtii CCAP 11/32A
L-glutamate + oxidized methylviologen
-
392019
Chlamydomonas reinhardtii CCAP 11/32A
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.4.1.14
30
-
assay at
Chlamydomonas reinhardtii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.1.14
additional information
-
cold-labile enzyme
Chlamydomonas reinhardtii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.1.14
7.5
-
-
Chlamydomonas reinhardtii
1.4.7.1
7.5
-
-
Chlamydomonas reinhardtii