Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Galvan, F.; Marquez, A.J.; Vega, J.M.
    Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardtii (1984), Planta, 162, 180-187.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.7.1 6-diazo-5-oxo-norleucine 5 mM, complete inhibition Chlamydomonas reinhardtii
1.4.7.1 azaserine 0.5 mM, complete inhibition Chlamydomonas reinhardtii
1.4.7.1 Bromocresol green 1 mM, 89% inhibition Chlamydomonas reinhardtii
1.4.7.1 Flavianate
-
 Chlamydomonas reinhardtii
1.4.7.1 N-ethylmaleimide 0.05 mM, 94% inhibition Chlamydomonas reinhardtii
1.4.7.1 p-hydroxymercuribenzoate
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.7.1 0.015
-
 reduced ferredoxin
-
 Chlamydomonas reinhardtii
1.4.7.1 0.7
-
 L-glutamine
-
 Chlamydomonas reinhardtii
1.4.7.1 1
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
1.4.7.1 4.7
-
 methyl viologen
-
 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.7.1 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.7.1 144000
-
 sucrose density gradient centrifugation Chlamydomonas reinhardtii
1.4.7.1 151000
-
 1 * 151000, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin Chlamydomonas reinhardtii highly specific for glutamine and 2-oxoglutarate L-glutamate + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.7.1 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.7.1 protamine sulfate, DEAE-Sephacel, hydroxylapatite, ferredoxin-Sepharose Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.7.1 leaf
-
 Chlamydomonas reinhardtii
-
1.4.7.1 root
-
 Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.7.1 10.4
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin highly specific for glutamine and 2-oxoglutarate Chlamydomonas reinhardtii L-glutamate + oxidized ferredoxin
-
 ?
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+
-
 Chlamydomonas reinhardtii L-glutamate + oxidized ferredoxin
-
 r
1.4.7.1 L-glutamine + 2-oxoglutarate + reduced methylviologen artificial elctron donor Chlamydomonas reinhardtii L-glutamate + oxidized methylviologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.7.1 monomer 1 * 151000, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.7.1 35
-
-
 Chlamydomonas reinhardtii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.7.1 35
-
-
 Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.7.1 50
-
 full activity after 10 min, 50% activity remains after heating at 55°C for 10 min, complete loss of activity at 60°C Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.7.1 7.5
-
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.7.1 flavin enzyme may contain a flavin prosthetic group Chlamydomonas reinhardtii