Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vianello, F.; Di Paolo, M.L.; Stevanato, R.; Gasparini, R.; Rigo, A.
    Purification and characterization of amine oxidase from soybean seedlings (1993), Arch. Biochem. Biophys., 307, 35-39.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.22 unstable at low ionic strenght, half-life of 8 d in 5 mM potassium phosphate, pH 7.0, at 4°C, 33 d if 100 mM NaCl is added, unstable if 0.01% n-octyl-beta-D-glucopyranoside or 0.1% Triton X-100 are added, loss of 25% activity after 5 d Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 1,4-diaminocyclohexane 5 mM, 69% inhibition Glycine max
1.4.3.22 aminoguanidine
-
 Glycine max
1.4.3.22 aniline 10 mM, 43% inhibition Glycine max
1.4.3.22 cyanide
-
 Glycine max
1.4.3.22 Cyclohexylamine 10 mM, 93% inhibition Glycine max
1.4.3.22 Dicyclohexylamine 10 mM, 79% inhibition Glycine max
1.4.3.22 diethyldithiocarbamate
-
 Glycine max
1.4.3.22 piperidine 10 mM, 55% inhibition Glycine max
1.4.3.22 Semicarbazide
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.065
-
 cadaverine
-
 Glycine max
1.4.3.22 0.19
-
 putrescine
-
 Glycine max
1.4.3.22 5.9
-
 spermidine
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 77000
-
 x * 77000, SDS-PAGE Glycine max
1.4.3.22 113000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Glycine max
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.22 glycoprotein heterogenity may be due to carbohydrate moiety Glycine max

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 seed
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 263
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
1.4.3.22 -80°C, 5 mM potassium phosphate, pH 7.0, 100 mM NaCl, 2 months, no loss of activity Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,4-diaminobutane + H2O + O2
-
 Glycine max 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 cadaverine + H2O + O2
-
 Glycine max 5-aminopentanal + NH3 + H2O2
-
 r
1.4.3.22 RCH2NH2 + H2O + O2
-
 Glycine max RCHO + NH3 + H2O2
-
 ?
1.4.3.22 spermidine + H2O + O2
-
 Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 ? x * 77000, SDS-PAGE Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.22 163
-
 putrescine
-
 Glycine max
1.4.3.22 193
-
 spermidine
-
 Glycine max
1.4.3.22 498
-
 cadaverine
-
 Glycine max

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.22 5 7 slight decrease in activity, complete loss at pH 4 and pH 8 Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.22 0.045
-
 Cyclohexylamine
-
 Glycine max
1.4.3.22 0.78
-
 Dicyclohexylamine
-
 Glycine max
1.4.3.22 1.2
-
 piperidine
-
 Glycine max
1.4.3.22 5
-
 aniline
-
 Glycine max