BRENDA - Enzyme Database

Purification and properties of diamine oxidase from human kidney

Suzuki, O.; Matsumoto, T.; Biogenic Amines 4, 237-245 (1987)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.4.3.22
unstable in dilute solution or in frozen state
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
8-hydroxyquinoline
1 mM, 49.3% inhibition
Homo sapiens
1.4.3.22
aminoguanidine
1 mM, complete inhibition
Homo sapiens
1.4.3.22
Cuprizone
-
Homo sapiens
1.4.3.22
cyanide
1 mM, 76% inhibition
Homo sapiens
1.4.3.22
diethyldithiocarbamate
1 mM, complete inhibition
Homo sapiens
1.4.3.22
additional information
not inhibited by pargyline
Homo sapiens
1.4.3.22
NaN3
-
Homo sapiens
1.4.3.22
Quinacrine
1 mM, complete inhibition
Homo sapiens
1.4.3.22
Semicarbazide
1 mM, complete inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.0172
-
histamine
-
Homo sapiens
1.4.3.22
0.0279
-
cadaverine
-
Homo sapiens
1.4.3.22
0.033
-
putrescine
-
Homo sapiens
1.4.3.22
0.625
-
N1-acetylspermidine
-
Homo sapiens
1.4.3.22
3.28
-
Acetylcadaverine
-
Homo sapiens
1.4.3.22
5.71
-
spermine
-
Homo sapiens
1.4.3.22
9.52
-
spermidine
-
Homo sapiens
1.4.3.22
9.71
-
acetylputrescine
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
105000
-
2 * 105000, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.22
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.22
affinity chromatography on AH-Sepharose
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
kidney
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.4.3.22
0.73
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.4.3.22
500 mM ammonium sulfate, 500 mM potassium phosphate, pH 7.4, stable for at least 3 weeks
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,3-diaminopropane + H2O + O2
109% of activity with putrescine
391947
Homo sapiens
3-aminopropanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391947
Homo sapiens
4-aminobutanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,5-diaminopentane + H2O + O2
most active
391947
Homo sapiens
5-aminopentanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391947
Homo sapiens
6-aminohexanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391947
Homo sapiens
7-aminoheptanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
agmatine
14% of activity with putrescine
391947
Homo sapiens
?
-
-
-
?
1.4.3.22
histamine + H2O + O2
28% of activity with putrescine
391947
Homo sapiens
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
N-acetyl-1,4-diaminobutane + H2O + O2
8.6% of activity with putrescine
391947
Homo sapiens
N-acetyl-aminobutanal + acetylamine + H2O2
-
391947
Homo sapiens
?
1.4.3.22
N-acetyl-1,5-diaminopentane + H2O + O2
3.8% of activity with putrescine
391947
Homo sapiens
N-acetyl-aminopentanal + acetylamine + H2O2
-
391947
Homo sapiens
?
1.4.3.22
N1-acetyl-spermidine + H2O + O2
8.6% of activity with putrescine
391947
Homo sapiens
?
-
-
-
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391947
Homo sapiens
RCHO + NH3 + H2O2
-
391947
Homo sapiens
?
1.4.3.22
spermidine + H2O + O2
-
391947
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 105000, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
6
6.5
histane
Homo sapiens
1.4.3.22
6.5
-
putrescine and cadaverine
Homo sapiens
1.4.3.22
8
-
N1-acetylspermidine
Homo sapiens
1.4.3.22
8.5
-
acetylcadaverine
Homo sapiens
1.4.3.22
9.5
-
spermidine and spermine
Homo sapiens
1.4.3.22
10
11
acetylputrescine
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.3.22
unstable in dilute solution or in frozen state
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
8-hydroxyquinoline
1 mM, 49.3% inhibition
Homo sapiens
1.4.3.22
aminoguanidine
1 mM, complete inhibition
Homo sapiens
1.4.3.22
Cuprizone
-
Homo sapiens
1.4.3.22
cyanide
1 mM, 76% inhibition
Homo sapiens
1.4.3.22
diethyldithiocarbamate
1 mM, complete inhibition
Homo sapiens
1.4.3.22
additional information
not inhibited by pargyline
Homo sapiens
1.4.3.22
NaN3
-
Homo sapiens
1.4.3.22
Quinacrine
1 mM, complete inhibition
Homo sapiens
1.4.3.22
Semicarbazide
1 mM, complete inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.0172
-
histamine
-
Homo sapiens
1.4.3.22
0.0279
-
cadaverine
-
Homo sapiens
1.4.3.22
0.033
-
putrescine
-
Homo sapiens
1.4.3.22
0.625
-
N1-acetylspermidine
-
Homo sapiens
1.4.3.22
3.28
-
Acetylcadaverine
-
Homo sapiens
1.4.3.22
5.71
-
spermine
-
Homo sapiens
1.4.3.22
9.52
-
spermidine
-
Homo sapiens
1.4.3.22
9.71
-
acetylputrescine
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
105000
-
2 * 105000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.22
affinity chromatography on AH-Sepharose
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
kidney
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.4.3.22
0.73
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.3.22
500 mM ammonium sulfate, 500 mM potassium phosphate, pH 7.4, stable for at least 3 weeks
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,3-diaminopropane + H2O + O2
109% of activity with putrescine
391947
Homo sapiens
3-aminopropanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391947
Homo sapiens
4-aminobutanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,5-diaminopentane + H2O + O2
most active
391947
Homo sapiens
5-aminopentanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391947
Homo sapiens
6-aminohexanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391947
Homo sapiens
7-aminoheptanal + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
agmatine
14% of activity with putrescine
391947
Homo sapiens
?
-
-
-
?
1.4.3.22
histamine + H2O + O2
28% of activity with putrescine
391947
Homo sapiens
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391947
Homo sapiens
-
1.4.3.22
N-acetyl-1,4-diaminobutane + H2O + O2
8.6% of activity with putrescine
391947
Homo sapiens
N-acetyl-aminobutanal + acetylamine + H2O2
-
391947
Homo sapiens
?
1.4.3.22
N-acetyl-1,5-diaminopentane + H2O + O2
3.8% of activity with putrescine
391947
Homo sapiens
N-acetyl-aminopentanal + acetylamine + H2O2
-
391947
Homo sapiens
?
1.4.3.22
N1-acetyl-spermidine + H2O + O2
8.6% of activity with putrescine
391947
Homo sapiens
?
-
-
-
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391947
Homo sapiens
RCHO + NH3 + H2O2
-
391947
Homo sapiens
?
1.4.3.22
spermidine + H2O + O2
-
391947
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 105000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
6
6.5
histane
Homo sapiens
1.4.3.22
6.5
-
putrescine and cadaverine
Homo sapiens
1.4.3.22
8
-
N1-acetylspermidine
Homo sapiens
1.4.3.22
8.5
-
acetylcadaverine
Homo sapiens
1.4.3.22
9.5
-
spermidine and spermine
Homo sapiens
1.4.3.22
10
11
acetylputrescine
Homo sapiens