BRENDA - Enzyme Database

Purification and properties of the diamine oxidase from Vicia faba leaves

Matsuda, H.; Suzuki, Y.; Plant Cell Physiol. 22, 737-746 (1981)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
1,4-phenanthroline
0.1 mM
Vicia faba
1.4.3.22
Cuprizone
0.1 mM
Vicia faba
1.4.3.22
diethyldithiocarbamate
-
Vicia faba
1.4.3.22
phenylhydrazine
-
Vicia faba
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
2 copper atoms per mol of enzyme
Vicia faba
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
74000
-
2 * 74000, SDS-PAGE
Vicia faba
1.4.3.22
126000
-
gel filtration
Vicia faba
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.22
Vicia faba
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.22
-
Vicia faba
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
leaf
-
Vicia faba
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.3.22
21.6
-
-
Vicia faba
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391941
Vicia faba
4-aminobutanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391941
Vicia faba
5-aminopentanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
2-phenylethylamine + H2O + O2
-
391941
Vicia faba
2-phenylethanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
agmatine
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
benzylamine + H2O + O2
-
391941
Vicia faba
benzaldehyde + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
histamine + H2O + O2
-
391941
Vicia faba
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
lysine + H2O + O2
-
391941
Vicia faba
2-aminohexanoic acid + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391941
Vicia faba
RCHO + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
spermidine + H2O + O2
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
tryptamine
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
tyramine + H2O + O2
-
391941
Vicia faba
4-hydroxyphenylethanal + NH3 + H2O2
-
391941
Vicia faba
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 74000, SDS-PAGE
Vicia faba
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.3.22
40
-
-
Vicia faba
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.4.3.22
20
63
about 50% activity at 20C and 63C
Vicia faba
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.4.3.22
-
60
10 min, pH 7.0, stable
Vicia faba
1.4.3.22
70
-
10 min, pH 7, 100% loss of activity
Vicia faba
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
cadaverine, histamine, agmatine
Vicia faba
1.4.3.22
7.2
-
putrescine, spermidine, tryptamine
Vicia faba
1.4.3.22
7.7
-
tyramine
Vicia faba
1.4.3.22
8.5
-
dopamine, phenylethylamine, benzylamine
Vicia faba
1.4.3.22
8.6
-
lysine
Vicia faba
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
3
-
4 h, complete loss of activity
Vicia faba
1.4.3.22
3.5
-
4 h, 50% loss of activity
Vicia faba
1.4.3.22
5
9
30C, stable
Vicia faba
1.4.3.22
11
-
4 h, 50% loss of activity
Vicia faba
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.22
additional information
1 mol carbonyl-group per mol enzyme
Vicia faba
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.22
additional information
1 mol carbonyl-group per mol enzyme
Vicia faba
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
1,4-phenanthroline
0.1 mM
Vicia faba
1.4.3.22
Cuprizone
0.1 mM
Vicia faba
1.4.3.22
diethyldithiocarbamate
-
Vicia faba
1.4.3.22
phenylhydrazine
-
Vicia faba
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
2 copper atoms per mol of enzyme
Vicia faba
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
74000
-
2 * 74000, SDS-PAGE
Vicia faba
1.4.3.22
126000
-
gel filtration
Vicia faba
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.22
-
Vicia faba
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
leaf
-
Vicia faba
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.4.3.22
21.6
-
-
Vicia faba
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391941
Vicia faba
4-aminobutanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391941
Vicia faba
5-aminopentanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
2-phenylethylamine + H2O + O2
-
391941
Vicia faba
2-phenylethanal + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
agmatine
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
benzylamine + H2O + O2
-
391941
Vicia faba
benzaldehyde + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
histamine + H2O + O2
-
391941
Vicia faba
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
lysine + H2O + O2
-
391941
Vicia faba
2-aminohexanoic acid + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391941
Vicia faba
RCHO + NH3 + H2O2
-
391941
Vicia faba
?
1.4.3.22
spermidine + H2O + O2
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
tryptamine
-
391941
Vicia faba
?
-
-
-
?
1.4.3.22
tyramine + H2O + O2
-
391941
Vicia faba
4-hydroxyphenylethanal + NH3 + H2O2
-
391941
Vicia faba
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 74000, SDS-PAGE
Vicia faba
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.4.3.22
40
-
-
Vicia faba
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.4.3.22
20
63
about 50% activity at 20C and 63C
Vicia faba
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.4.3.22
-
60
10 min, pH 7.0, stable
Vicia faba
1.4.3.22
70
-
10 min, pH 7, 100% loss of activity
Vicia faba
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
cadaverine, histamine, agmatine
Vicia faba
1.4.3.22
7.2
-
putrescine, spermidine, tryptamine
Vicia faba
1.4.3.22
7.7
-
tyramine
Vicia faba
1.4.3.22
8.5
-
dopamine, phenylethylamine, benzylamine
Vicia faba
1.4.3.22
8.6
-
lysine
Vicia faba
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
3
-
4 h, complete loss of activity
Vicia faba
1.4.3.22
3.5
-
4 h, 50% loss of activity
Vicia faba
1.4.3.22
5
9
30C, stable
Vicia faba
1.4.3.22
11
-
4 h, 50% loss of activity
Vicia faba