BRENDA - Enzyme Database

Amine oxidase from Lathyrus cicera and Phaseolus vulgaris: purification and properties

Cogoni, A.; Farci, R.; Medda, R.; Rinaldi, A.; Floris, G.; Prep. Biochem. 19, 95-112 (1989)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.4.3.22
enzyme does not withstand lyophilization
Phaseolus vulgaris
1.4.3.22
enzyme does not withstand lyophilization
Lathyrus cicera
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
beta-Bromoethylamine
-
Lathyrus cicera
1.4.3.22
beta-Bromoethylamine
-
Phaseolus vulgaris
1.4.3.22
diethyldithiocarbamate
-
Lathyrus cicera
1.4.3.22
diethyldithiocarbamate
-
Phaseolus vulgaris
1.4.3.22
NaCN
-
Lathyrus cicera
1.4.3.22
NaCN
-
Phaseolus vulgaris
1.4.3.22
phenylhydrazine
-
Lathyrus cicera
1.4.3.22
phenylhydrazine
-
Phaseolus vulgaris
1.4.3.22
Semicarbazide
-
Lathyrus cicera
1.4.3.22
Semicarbazide
-
Phaseolus vulgaris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.32
-
putrescine
-
Lathyrus cicera
1.4.3.22
7
-
putrescine
-
Phaseolus vulgaris
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
enzyme contains 0.082% copper
Lathyrus cicera
1.4.3.22
copper
enzyme contains 0.082% copper
Phaseolus vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
75000
-
2 * 75000, SDS-PAGE
Lathyrus cicera
1.4.3.22
75000
-
2 * 75000, SDS-PAGE
Phaseolus vulgaris
1.4.3.22
150000
-
gel filtration
Lathyrus cicera
1.4.3.22
150000
-
gel filtration
Phaseolus vulgaris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.22
Lathyrus cicera
-
-
-
1.4.3.22
Phaseolus vulgaris
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.4.3.22
glycoprotein
14% neutral sugar
Lathyrus cicera
1.4.3.22
glycoprotein
14% neutral sugar
Phaseolus vulgaris
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.22
-
Phaseolus vulgaris
1.4.3.22
-
Lathyrus cicera
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
seed
-
Lathyrus cicera
-
1.4.3.22
seed
-
Phaseolus vulgaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.22
6.7
-
-
Phaseolus vulgaris
1.4.3.22
50.8
-
-
Lathyrus cicera
Storage Stability
EC Number
Storage Stability
Organism
1.4.3.22
-20°C, pH 7, indefinitely stable
Phaseolus vulgaris
1.4.3.22
-20°C, pH 7, indefinitely stable
Lathyrus cicera
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391940
Phaseolus vulgaris
4-aminobutanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391940
Lathyrus cicera
4-aminobutanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391940
Phaseolus vulgaris
5-aminopentanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391940
Lathyrus cicera
5-aminopentanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391940
Phaseolus vulgaris
6-aminohexanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391940
Lathyrus cicera
6-aminohexanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391940
Phaseolus vulgaris
7-aminoheptanal + NH3 + H2O2
-
-
-
?
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391940
Lathyrus cicera
7-aminoheptanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
agmatine
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
agmatine
-
391940
Lathyrus cicera
?
-
-
-
?
1.4.3.22
p-dimethylaminomethylbenzylamine + H2O + O2
-
391940
Phaseolus vulgaris
p-dimethylaminomethylbenzaldehyde + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
p-dimethylaminomethylbenzylamine + H2O + O2
-
391940
Lathyrus cicera
p-dimethylaminomethylbenzaldehyde + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391940
Phaseolus vulgaris
RCHO + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391940
Lathyrus cicera
RCHO + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
spermidine + H2O + O2
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
spermidine + H2O + O2
-
391940
Lathyrus cicera
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391940
Lathyrus cicera
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 75000, SDS-PAGE
Lathyrus cicera
1.4.3.22
dimer
2 * 75000, SDS-PAGE
Phaseolus vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
-
Lathyrus cicera
1.4.3.22
7.5
-
-
Phaseolus vulgaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.22
pyrroloquinoline quinone
enzyme may contain pyrroloquinoline-quinione as prosthetic group
Lathyrus cicera
1.4.3.22
pyrroloquinoline quinone
enzyme may contain pyrroloquinoline-quinione as prosthetic group
Phaseolus vulgaris
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.22
0.00004
-
phenylhydrazine
-
Phaseolus vulgaris
1.4.3.22
0.00006
-
phenylhydrazine
-
Lathyrus cicera
1.4.3.22
0.0027
-
beta-Bromoethylamine
-
Phaseolus vulgaris
1.4.3.22
0.003
-
Semicarbazide
-
Phaseolus vulgaris
1.4.3.22
0.0063
-
Semicarbazide
-
Lathyrus cicera
1.4.3.22
0.007
-
beta-Bromoethylamine
-
Lathyrus cicera
1.4.3.22
0.07
-
diethyldithiocarbamate
-
Lathyrus cicera
1.4.3.22
0.09
-
diethyldithiocarbamate
-
Phaseolus vulgaris
1.4.3.22
0.3
-
NaCN
-
Lathyrus cicera
1.4.3.22
0.83
-
NaCN
-
Phaseolus vulgaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.22
pyrroloquinoline quinone
enzyme may contain pyrroloquinoline-quinione as prosthetic group
Lathyrus cicera
1.4.3.22
pyrroloquinoline quinone
enzyme may contain pyrroloquinoline-quinione as prosthetic group
Phaseolus vulgaris
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.3.22
enzyme does not withstand lyophilization
Phaseolus vulgaris
1.4.3.22
enzyme does not withstand lyophilization
Lathyrus cicera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
beta-Bromoethylamine
-
Lathyrus cicera
1.4.3.22
beta-Bromoethylamine
-
Phaseolus vulgaris
1.4.3.22
diethyldithiocarbamate
-
Lathyrus cicera
1.4.3.22
diethyldithiocarbamate
-
Phaseolus vulgaris
1.4.3.22
NaCN
-
Lathyrus cicera
1.4.3.22
NaCN
-
Phaseolus vulgaris
1.4.3.22
phenylhydrazine
-
Lathyrus cicera
1.4.3.22
phenylhydrazine
-
Phaseolus vulgaris
1.4.3.22
Semicarbazide
-
Lathyrus cicera
1.4.3.22
Semicarbazide
-
Phaseolus vulgaris
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.4.3.22
0.00004
-
phenylhydrazine
-
Phaseolus vulgaris
1.4.3.22
0.00006
-
phenylhydrazine
-
Lathyrus cicera
1.4.3.22
0.0027
-
beta-Bromoethylamine
-
Phaseolus vulgaris
1.4.3.22
0.003
-
Semicarbazide
-
Phaseolus vulgaris
1.4.3.22
0.0063
-
Semicarbazide
-
Lathyrus cicera
1.4.3.22
0.007
-
beta-Bromoethylamine
-
Lathyrus cicera
1.4.3.22
0.07
-
diethyldithiocarbamate
-
Lathyrus cicera
1.4.3.22
0.09
-
diethyldithiocarbamate
-
Phaseolus vulgaris
1.4.3.22
0.3
-
NaCN
-
Lathyrus cicera
1.4.3.22
0.83
-
NaCN
-
Phaseolus vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.32
-
putrescine
-
Lathyrus cicera
1.4.3.22
7
-
putrescine
-
Phaseolus vulgaris
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
enzyme contains 0.082% copper
Lathyrus cicera
1.4.3.22
copper
enzyme contains 0.082% copper
Phaseolus vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
75000
-
2 * 75000, SDS-PAGE
Lathyrus cicera
1.4.3.22
75000
-
2 * 75000, SDS-PAGE
Phaseolus vulgaris
1.4.3.22
150000
-
gel filtration
Lathyrus cicera
1.4.3.22
150000
-
gel filtration
Phaseolus vulgaris
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.4.3.22
glycoprotein
14% neutral sugar
Lathyrus cicera
1.4.3.22
glycoprotein
14% neutral sugar
Phaseolus vulgaris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.22
-
Phaseolus vulgaris
1.4.3.22
-
Lathyrus cicera
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.3.22
seed
-
Lathyrus cicera
-
1.4.3.22
seed
-
Phaseolus vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.22
6.7
-
-
Phaseolus vulgaris
1.4.3.22
50.8
-
-
Lathyrus cicera
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.3.22
-20°C, pH 7, indefinitely stable
Phaseolus vulgaris
1.4.3.22
-20°C, pH 7, indefinitely stable
Lathyrus cicera
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391940
Phaseolus vulgaris
4-aminobutanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,4-diaminobutane + H2O + O2
-
391940
Lathyrus cicera
4-aminobutanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391940
Phaseolus vulgaris
5-aminopentanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,5-diaminopentane + H2O + O2
-
391940
Lathyrus cicera
5-aminopentanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391940
Phaseolus vulgaris
6-aminohexanal + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
1,6-diaminohexane + H2O + O2
-
391940
Lathyrus cicera
6-aminohexanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391940
Phaseolus vulgaris
7-aminoheptanal + NH3 + H2O2
-
-
-
?
1.4.3.22
1,7-diaminoheptane + H2O + O2
-
391940
Lathyrus cicera
7-aminoheptanal + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
agmatine
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
agmatine
-
391940
Lathyrus cicera
?
-
-
-
?
1.4.3.22
p-dimethylaminomethylbenzylamine + H2O + O2
-
391940
Phaseolus vulgaris
p-dimethylaminomethylbenzaldehyde + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
p-dimethylaminomethylbenzylamine + H2O + O2
-
391940
Lathyrus cicera
p-dimethylaminomethylbenzaldehyde + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391940
Phaseolus vulgaris
RCHO + NH3 + H2O2
-
391940
Phaseolus vulgaris
?
1.4.3.22
RCH2NH2 + H2O + O2
-
391940
Lathyrus cicera
RCHO + NH3 + H2O2
-
391940
Lathyrus cicera
?
1.4.3.22
spermidine + H2O + O2
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
spermidine + H2O + O2
-
391940
Lathyrus cicera
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391940
Phaseolus vulgaris
?
-
-
-
?
1.4.3.22
spermine + H2O + O2
-
391940
Lathyrus cicera
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.22
dimer
2 * 75000, SDS-PAGE
Lathyrus cicera
1.4.3.22
dimer
2 * 75000, SDS-PAGE
Phaseolus vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
7
-
-
Lathyrus cicera
1.4.3.22
7.5
-
-
Phaseolus vulgaris