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Literature summary extracted from

  • Yanagisawa, H.; Hirasawa, E.; Suzuki, Y.
    Purification and properties of diamine oxidase from pea epicotyls (1981), Phytochemistry, 20, 2105-2108.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.22 methylglyoxalbis(guanylhydrazone) without preincubation competitive inhibitor, non-competitive after 1 h preincubation Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 85000
-
 2 * 85000, SDS-PAGE Pisum sativum
1.4.3.22 180000
-
-
 Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22 phosphocellulose, methylglyoxalbis(guanylhydrazone)-Sepharose Pisum sativum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 69.62
-
 enzyme from epicotyl Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 RCH2NH2 + H2O + O2
-
 Pisum sativum RCHO + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 85000, SDS-PAGE Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.22 additional information contains one carbonyl-like group per mol Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.22 0.017
-
 methylglyoxalbis(guanylhydrazone) after preincubation for 1 h Pisum sativum
1.4.3.22 0.26
-
 methylglyoxalbis(guanylhydrazone) without preincubation Pisum sativum