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Literature summary extracted from

  • Tsuge, H.; Ozeki, K.; Ohashi, K.
    Molecular and enzymatic properties of pyridoxamine (pyridoxine) 5'-phosphate oxidase from baker's yeast (1980), Agric. Biol. Chem., 44, 2329-2335.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.000016
-
 FMN
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.5 25000
-
 1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae
1.4.3.5 27000
-
 1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae
1.4.3.5 55000
-
 analytical polyacrylamide disc gel electrophoresis Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.0417
-
 activity of apoenzyme after dialysis of holoenzyme against potassium acetate buffer pH 4.0 and potassium phosphate buffer pH 7.0 for 24 h each Saccharomyces cerevisiae
1.4.3.5 0.435
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 dimer 1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN 1 mol of FMN per mol of enzyme Saccharomyces cerevisiae