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Literature summary extracted from

  • Kazarinoff, M.N.; McCormick, D.B.
    N-(5-phospho-4-pyridoxyl)amines as substrates for pyridoxine (pyridoxamine) 5-phosphate oxidase (1973), Biochem. Biophys. Res. Commun., 52, 440-446.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.0013
-
 pyridoxamine 5'-phosphate pH 8.0 Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5
-
 Oryctolagus cuniculus
1.4.3.5 native enzyme 556fold from liver by acid and ethanol precipitations, anion exchange chromatography, gel filtration, and calcium phosphate gel chromatography Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2 reaction mechanism Oryctolagus cuniculus
a
1.4.3.5 pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2 reaction mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.156
-
-
 Oryctolagus cuniculus
1.4.3.5 0.157
-
 purified enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 5'-phospho-pyridoxyl-2-aminobutyrate + H2O + O2 110% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + 2-aminobutyrate + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-3-alanine + H2O + O2 85% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + alanine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-benzylamine + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + benzylamine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-D-2-aminobutyrate + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-2-aminobutyrate + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-D-alanine + H2O + O2 130% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-alanine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-D-leucine + H2O + O2 120% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-leucine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-D-tyrosine + H2O + O2 55% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + D-tyrosine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-glycine + H2O + O2 110% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + glycine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-2-aminobutyrate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + L-2-aminobutyrate + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-alanine + H2O + O2 140% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-alanine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-leucine + H2O + O2 86% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-leucine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-phenylalanine + H2O + O2 54% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-phenylalanine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-serine + H2O + O2 39% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-serine + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-tryptophan + H2O + O2 18% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tryptophan + H2O2
-
 ?
1.4.3.5 5'-phospho-pyridoxyl-L-tyrosine + H2O + O2 57% of activity with pyridoxamine 5'-phosphate Oryctolagus cuniculus pyridoxal 5'-phosphate + L-tyrosine + H2O2
-
 ?
1.4.3.5 additional information a 5'-phosphate moiety is absolutely required for activity, substrate specificities with various N-(phosphopyridoxyl)amines, overview, no activity with pyridoxamine, N-(pyridoxyl)-glycine, N-(pyridoxyl)-S-alanine, or N-(pyridoxyl)-L-tyrosine Oryctolagus cuniculus ?
-
 ?
1.4.3.5 N10-(5'-phosphopyridoxyl)-1,10-diaminodecane + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + 1,10-diaminodecane + H2O2
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 8
-
 assay at Oryctolagus cuniculus