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Literature summary extracted from

  • Tsuge, H.; Kuroda, Y.; Iwamoto, A.; Ohashi, K.
    Partial purification and property of pyridoxine (pyridoxamine)-5-phosphate oxidase isozymes from wheat seedlings (1982), Arch. Biochem. Biophys., 217, 479-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.5 ammonium sulfate short incubation activates isozyme E-II, not E-I Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 ammonium sulfate 1.9 M, E II: activation at short incubation time, E I: complete inactivation after 10 h incubation; inactivation of isozyme E-I after 10 h Triticum aestivum
1.4.3.5 Cd2+ 0.5 mM, isozyme E I: insensitive, isozyme E II: 44% inhibition; 44% reduced activity of isozyme E-II, no inhibition of isozyme E-I Triticum aestivum
1.4.3.5 citrate-phosphate buffer
-
 Triticum aestivum
1.4.3.5 Hg2+ 0.5 mM, isozyme E I: insensitive, isozyme E II: 54% inhibition; 72% reduced activity of isozyme E-II, no inhibition of isozyme E-I Triticum aestivum
1.4.3.5 Zn2+ 0.5 mM, isozyme E I: insensitive, isozyme E II: 54% inhibition; 72% reduced activity of isozyme E-II, no inhibition of isozyme E-I Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.0067
-
 pyridoxamine 5'-phosphate isozyme E-II, pH 8.0, 37°C Triticum aestivum
1.4.3.5 0.008
-
 pyridoxine 5'-phosphate isozyme E-II, pH 8.0, 37°C Triticum aestivum
1.4.3.5 0.02
-
 pyridoxine 5'-phosphate isozyme E-I, pH 8.0, 37°C Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Triticum aestivum
-
 isozymes: E1 and E2
-
1.4.3.5 Triticum aestivum
-
 two isozymes E-I and E-II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 two isozymes from seedlings, partially by anion exchange and adsorption chromatography Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 seedling
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.00188
-
 isozyme EII Triticum aestivum
1.4.3.5 0.00367
-
 isozyme EI Triticum aestivum
1.4.3.5 0.113
-
 purified isozyme E-II, substrate pyridoxine 5'-phosphate Triticum aestivum
1.4.3.5 0.45
-
 purified isozyme E-I, substrate pyridoxine 5'-phosphate Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ 1.6fold preferred substrate of isozyme E-II Triticum aestivum pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 E II uses pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate Triticum aestivum pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 isozymes E I uses only pyridoxine 5'-phosphate as substrate Triticum aestivum pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN 9fold preferred substrate of isozyme E-I Triticum aestivum pyridoxal 5'-phosphate + FMNH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.5 37
-
 assay at Triticum aestivum
1.4.3.5 40
-
 isozymes EI and EII, rapid decrease above Triticum aestivum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.5 additional information
-
 rapid decrease in activity above 40°C, both isozymes Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.5 9.5
-
-
 Triticum aestivum
1.4.3.5 9.5
-
 both isozymes Triticum aestivum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.5 6.1
-
 37°C, 30 min, isozyme E I: 85% loss of activity, isozyme E II: 50% loss of activity Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.5 FMN
-
 Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.5 Triticum aestivum the pI values of the isozymes differ from each other
-
 additional information