Literature summary extracted from

Pilone, M.S.
D-Amino acid oxidase: new findings (2000), Cell. Mol. Life Sci., 57, 1732-1747.

Application

EC Number	Application	Comment	Organism
1.4.3.3	medicine	human gene therapy	Rhodotorula toruloides
1.4.3.3	medicine	human gene therapy	Trigonopsis variabilis
1.4.3.3	synthesis	oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid	Rhodotorula toruloides
1.4.3.3	synthesis	oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid	Trigonopsis variabilis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.4.3.3	-	Rhodotorula toruloides
1.4.3.3	expression in Kluyveromyces lactis	Trigonopsis variabilis
1.4.3.3	expression in Saccharomyces cerevisiae	Trigonopsis variabilis

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.4.3.3	Y223F	slower substrate binding than the wild-type	Trigonopsis variabilis

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.4.3.3	40000	-	alpha2, 2 * 40000, SDS-PAGE	Trigonopsis variabilis
1.4.3.3	79000	-	-	Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.4.3.3	cephalosporin C + H2O + O2	Rhodotorula toruloides	-	7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2	-	?
1.4.3.3	cephalosporin C + H2O + O2	Trigonopsis variabilis	-	7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.3.3	Homo sapiens	-	-	-
1.4.3.3	Rhodotorula toruloides	-	-	-
1.4.3.3	Sus scrofa	-	-	-
1.4.3.3	Trigonopsis variabilis	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.4.3.3	kidney	-	Homo sapiens	-
1.4.3.3	kidney	-	Sus scrofa	-
1.4.3.3	liver	-	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.3.3	cephalosporin C + H2O + O2	-	Rhodotorula toruloides	7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2	-	?
1.4.3.3	cephalosporin C + H2O + O2	-	Trigonopsis variabilis	7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.4.3.3	dimer	-	Rhodotorula toruloides
1.4.3.3	dimer	alpha2, 2 * 40000, SDS-PAGE	Trigonopsis variabilis
1.4.3.3	monomer	-	Sus scrofa

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4.3.3	0.05	-	D-alanine	-	Rhodotorula toruloides
1.4.3.3	0.8	-	D-alanine	-	Rhodotorula toruloides
1.4.3.3	4.2	-	D-alanine	mutant Y223S	Rhodotorula toruloides
1.4.3.3	10	-	D-alanine	-	Homo sapiens
1.4.3.3	10	-	D-alanine	-	Sus scrofa
1.4.3.3	210	-	D-alanine	mutant Y223F	Rhodotorula toruloides
1.4.3.3	345	-	D-alanine	-	Rhodotorula toruloides
1.4.3.3	350	-	D-alanine	-	Rhodotorula toruloides
1.4.3.3	737	-	cephalosporin C	-	Rhodotorula toruloides

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.3.3	FAD	flavoprotein	Homo sapiens
1.4.3.3	FAD	flavoprotein	Sus scrofa
1.4.3.3	FAD	flavoprotein	Rhodotorula toruloides
1.4.3.3	FAD	flavoprotein	Trigonopsis variabilis

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Release 2023.1 (January 2023)