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Literature summary extracted from

  • Pollegioni, L.; Fukui, K.; Massey, V.
    Studies on the kinetic mechanism of pig kidney D-amino acid oxidase by site-directed mutagenesis of tyrosine 224 and tyrosine 228 (1994), J. Biol. Chem., 269, 31666-31673.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3
-
 Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.3 Y224F turnover numbers similar to wild-type Sus scrofa
1.4.3.3 Y228F turnover numbers lower than turnover numbers of wild-type Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 2.3
-
 D-alanine wild-type Sus scrofa
1.4.3.3 2.8
-
 D-alanine mutant Y224F Sus scrofa
1.4.3.3 7.5
-
 D-phenylalanine mutant Y224F Sus scrofa
1.4.3.3 9.1
-
 D-proline mutant Y224F Sus scrofa
1.4.3.3 9.5
-
 D-proline wild-type Sus scrofa
1.4.3.3 12.2
-
 D-proline mutant Y228F Sus scrofa
1.4.3.3 15.6
-
 D-methionine mutant Y224F Sus scrofa
1.4.3.3 18.3
-
 D-phenylalanine mutant Y228F Sus scrofa
1.4.3.3 27
-
 D-alanine mutant Y228F Sus scrofa
1.4.3.3 50
-
 D-methionine mutant Y228F Sus scrofa
1.4.3.3 55.9
-
 D-phenylalanine wild-type Sus scrofa
1.4.3.3 143
-
 D-methionine wild-type Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Sus scrofa
-
 pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 Sus scrofa
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 Sus scrofa
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2 Sus scrofa
-
 2-oxopentanoic acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 0.15
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 Sus scrofa pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Sus scrofa 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Sus scrofa phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 Sus scrofa 2-oxopentanoic acid + NH3 + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 5
-
 D-alanine mutant Y228F Sus scrofa
1.4.3.3 7.1
-
 D-phenylalanine mutant Y228F Sus scrofa
1.4.3.3 11.6
-
 D-alanine mutant Y224F Sus scrofa
1.4.3.3 11.7
-
 D-proline mutant Y228F Sus scrofa
1.4.3.3 12.7
-
 D-alanine wild-type Sus scrofa
1.4.3.3 47.5
-
 D-methionine mutant Y228F Sus scrofa
1.4.3.3 48.8
-
 D-phenylalanine mutant Y224F Sus scrofa
1.4.3.3 55.9
-
 D-phenylalanine wild-type Sus scrofa
1.4.3.3 83.7
-
 D-proline mutant Y224F Sus scrofa
1.4.3.3 87.2
-
 D-proline wild-type Sus scrofa
1.4.3.3 92
-
 D-methionine mutant Y224F Sus scrofa
1.4.3.3 143
-
 D-methionine wild-type Sus scrofa