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Literature summary extracted from

  • Rosenfeld, M.G.; Leiter, E.H.
    Isolation and characterization of a mitochondrial D-amino acid oxidase from Neurospora crassa (1977), Can. J. Biochem., 55, 66-74.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 Hg2+
-
 Neurospora crassa
1.4.3.3 p-chloromercuribenzoate
-
 Neurospora crassa
1.4.3.3 Zn2+
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.24
-
 D-methionine
-
 Neurospora crassa
1.4.3.3 0.28
-
 D-leucine
-
 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.3 mitochondrion matrix Neurospora crassa 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 118000 125000 PAGE, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Neurospora crassa
-
 pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-ethionine + H2O + O2 Neurospora crassa
-
 4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2 Neurospora crassa
-
 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 Neurospora crassa
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-norleucine + H2O + O2 Neurospora crassa
-
 2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-norvaline + H2O + O2 Neurospora crassa
-
 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 Neurospora crassa
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2 Neurospora crassa
-
 alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 partial Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 0.5
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 Neurospora crassa pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-ethionine + H2O + O2
-
 Neurospora crassa 4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 Neurospora crassa 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Neurospora crassa 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-norleucine + H2O + O2
-
 Neurospora crassa 2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-norvaline + H2O + O2
-
 Neurospora crassa 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Neurospora crassa phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2
-
 Neurospora crassa alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 20 30
-
 Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 9 9.2 D-methionine Neurospora crassa