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Literature summary extracted from

  • Berg, C.P.; Rodden, F.A.
    Purification of D-amino oxidase from Trigonopsis variabilis (1976), Anal. Biochem., 71, 214-222.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.3 benzoic acid stabilizes Trigonopsis variabilis
1.4.3.3 m-toluic acid stabilizes Trigonopsis variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 p-chloromercuribenzoate
-
 Trigonopsis variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.28
-
 D-leucine
-
 Trigonopsis variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-histidine + H2O + O2 Trigonopsis variabilis
-
 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2 Trigonopsis variabilis
-
 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 Trigonopsis variabilis
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tryptophan + H2O + O2 Trigonopsis variabilis
-
 indol-3-pyruvic acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Trigonopsis variabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Trigonopsis variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-histidine + H2O + O2
-
 Trigonopsis variabilis 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 Trigonopsis variabilis 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Trigonopsis variabilis 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tryptophan + H2O + O2
-
 Trigonopsis variabilis indol-3-pyruvic acid + NH3 + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 52
-
 pH 5.2, addition of benzoic acid or m-toluic acid, 7 min stable Trigonopsis variabilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD tightly bound Trigonopsis variabilis
1.4.3.3 FAD flavoprotein Trigonopsis variabilis