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Literature summary extracted from

  • Nakano, M.; Danowski, T.S.
    L-Amino acid oxidase (rat kidney) (1971), Methods Enzymol., 17B, 601-605.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.2
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.2 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 310000 314000 high speed equilibrium method, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.2 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.4.3.2 0-3°C, dialyzed enzyme, 2 weeks, 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2
-
 Rattus norvegicus 2-oxo acid + NH3 + H2O2
-
 ?
1.4.3.2 L-lactic acid + H2O + O2
-
 Rattus norvegicus pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-leucine + H2O + O2
-
 Rattus norvegicus 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 additional information not: alpha-hydroxyisobutyrate Rattus norvegicus ?
-
 ?
1.4.3.2 additional information not: L-threonine Rattus norvegicus ?
-
 ?
1.4.3.2 thyroxine + H2O + O2
-
 Rattus norvegicus 3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenylpyruvate] + NH3 + H2O2
-
 ?
1.4.3.2 triiodothyronine + H2O + O2
-
 Rattus norvegicus 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropionic acid + NH3 + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 6.3
-
 L-leucine
-
 Rattus norvegicus
1.4.3.2 26
-
 L-lactic acid
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 8
-
 L-lactic acid Rattus norvegicus
1.4.3.2 8.7
-
 L-leucine Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FMN 6 mol of FMN per mol of enzyme Rattus norvegicus