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Literature summary extracted from

  • Sakuraba, H.; Satomura, T.; Kawakami, R.; Yamamoto, S.; Kawarabayasi, Y.; Kikuchi, H.; Ohshima, T.
    L-Aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing (2002), Extremophiles, 6, 275-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.16 stable at 4°C for at least 2 months without loss of activity Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.16 51000
-
 3 * 51000, SDS-PAGE Pyrococcus horikoshii
1.4.3.16 151000
-
 gel filtration Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.16 Pyrococcus horikoshii
-
 OT-3
-
1.4.3.16 Pyrococcus horikoshii OT-3
-
 OT-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.16 overexpression in Escherichia coli Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.16 3.2
-
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.16 L-aspartate + H2O + O2 2,6-dichlorophenol-indophenol, ferricyanide and fumarate can be electron acceptor Pyrococcus horikoshii oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.16 L-aspartate + H2O + O2 2,6-dichlorophenol-indophenol, ferricyanide and fumarate can be electron acceptor Pyrococcus horikoshii OT-3 oxaloacetate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.16 trimer 3 * 51000, SDS-PAGE Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.16 90
-
-
 Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.16 8.6
-
-
 Pyrococcus horikoshii