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Literature summary extracted from

  • Weber, E.; Tonder, K.; Reinbothe, C.; Unverhau, K.; Weide, H.; Aurich, H.
    L-Lysine alpha-oxidase from Trichoderma viride i4. Purification and characterization (1994), J. Basic Microbiol., 34, 265-276.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.14 1,4-diaminobutane 20% inhibition Trichoderma viride
1.4.3.14 1,5-Diaminopentane 24% inhibition Trichoderma viride
1.4.3.14 1,6-diaminohexane 43% inhibition Trichoderma viride
1.4.3.14 6-aminocaproic acid 30% inhibition Trichoderma viride
1.4.3.14 CoSO4
-
 Trichoderma viride
1.4.3.14 CuCl2
-
 Trichoderma viride
1.4.3.14 HgCl2
-
 Trichoderma viride
1.4.3.14 p-chloromercuribenzoate
-
 Trichoderma viride
1.4.3.14 ZnSO4
-
 Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.14 0.026
-
 L-lysine
-
 Trichoderma viride
1.4.3.14 0.625
-
 L-ornithine
-
 Trichoderma viride
1.4.3.14 0.68
-
 L-arginine
-
 Trichoderma viride

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.14 55000
-
 2 * 55000, SDS-PAGE Trichoderma viride
1.4.3.14 110000
-
 gel filtration Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride preferred substrate 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O Trichoderma viride i4 preferred substrate 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.4.3.14 Acetone 60%, complete inactivation after 60 min Trichoderma viride
1.4.3.14 Ethanol 70%, immediately inactivated Trichoderma viride

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.14 Trichoderma viride
-
 i4
-
1.4.3.14 Trichoderma viride i4
-
 i4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.14 acetone precipitation, ion-exchange Trichoderma viride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.14 90
-
-
 Trichoderma viride

Storage Stability

EC Number Storage Stability Organism
1.4.3.14 -20°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, at least 14 months stable without any loss of activity Trichoderma viride
1.4.3.14 4-37°C, 0.05 M potassium phosphate buffer of Tris-HCl buffer pH 8.0, stable for 4-5 days Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.14 L-arginine + O2 + H2O
-
 Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-arginine + O2 + H2O
-
 Trichoderma viride i4 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-histidine + O2 + H2O only 1% in comparison with L-lysine Trichoderma viride 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O preferred substrate Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-lysine + O2 + H2O preferred substrate Trichoderma viride i4 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.14 L-ornithine + O2 + H2O
-
 Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.14 L-ornithine + O2 + H2O
-
 Trichoderma viride i4 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.14 L-phenylalanine + O2 + H2O 16% in comparison to L-lysine Trichoderma viride phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.14 L-phenylalanine + O2 + H2O 16% in comparison to L-lysine Trichoderma viride i4 phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.14 additional information several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested Trichoderma viride ?
-
 ?
1.4.3.14 additional information several amino acids, amino acid derivatives and other substances that are assumed to be possible substrates are tested Trichoderma viride i4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.14 dimer 2 * 55000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.14 50
-
-
 Trichoderma viride

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.14 additional information
-
 stable at relatively high temperatures Trichoderma viride
1.4.3.14 30
-
 30 min, 0.1 M potassium phosphate buffer, reaction rate is between 85% at pH 3.0 and 90% at pH 11.0 Trichoderma viride
1.4.3.14 70
-
 total loss of activity if preincubated at 70°C Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.14 8 9
-
 Trichoderma viride

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.14 5 11 enzyme activity does not fall below 60%, at pH 3.0 the activity is still 30% Trichoderma viride

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.14 additional information
-
 stable at extreme pH values Trichoderma viride

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.14 FAD 1 mol per subunit, non-covalently bound Trichoderma viride