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Literature summary extracted from

  • Kamei, T.; Asano, K.; Kondo, H.; Matsuzaki, M.; Nakamura, S.
    L-Glutamate oxidase from Streptomyces violascens. II. Properties (1983), Chem. Pharm. Bull., 31, 3609-3616.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.3.11 analysis specific and sensitive determination of L-glutamate Streptomyces violascens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.11 Ag+ 1 mM AgNO3, complete inhibition Streptomyces violascens
1.4.3.11 Cu2+ 1 mM CuCl2, 22.4% inhibition Streptomyces violascens
1.4.3.11 Hg2+ 1 mM HgCl2, complete inhibition Streptomyces violascens
1.4.3.11 N-bromosuccinimide 1 mM, 77.6% inhibition Streptomyces violascens
1.4.3.11 PCMB 1 mM, 42.8% inhibition Streptomyces violascens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.11 1.1
-
 L-glutamate pH 5.0 Streptomyces violascens
1.4.3.11 3.3
-
 L-glutamate pH 6.8 Streptomyces violascens
1.4.3.11 5
-
 L-His pH 6.8 Streptomyces violascens
1.4.3.11 6.7
-
 L-Gln pH 6.8 Streptomyces violascens
1.4.3.11 10
-
 L-Gln pH 5.0 Streptomyces violascens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.11 Streptomyces violascens
-
 H82-N-SY7
-
1.4.3.11 Streptomyces violascens H82-N-SY7
-
 H82-N-SY7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.11 L-Gln + O2 + H2O 2.5% of the activity with L-glutamate at pH 5.0, 32.1% of the activity with L-glutamate at pH 6.8 Streptomyces violascens 2-oxoglutaric acid 5-amide + NH3 + H2O2
-
 ?
1.4.3.11 L-Gln + O2 + H2O 2.5% of the activity with L-glutamate at pH 5.0, 32.1% of the activity with L-glutamate at pH 6.8 Streptomyces violascens H82-N-SY7 2-oxoglutaric acid 5-amide + NH3 + H2O2
-
 ?
1.4.3.11 L-glutamate + O2 + H2O no activity with NADP+, methylene blue, thionine and ferricyanide as electron acceptor Streptomyces violascens 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.11 L-glutamate + O2 + H2O no activity with NADP+, methylene blue, thionine and ferricyanide as electron acceptor Streptomyces violascens H82-N-SY7 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.11 L-His + O2 + H2O 2.4% of the activity with L-glutamate, 13.1% of the activity with L-glutamate at pH 6.8 Streptomyces violascens 2-oxo-3-(1H-imidazol-4yl)-propionic acid + NH3 + H2O2
-
 ?
1.4.3.11 L-His + O2 + H2O 2.4% of the activity with L-glutamate, 13.1% of the activity with L-glutamate at pH 6.8 Streptomyces violascens H82-N-SY7 2-oxo-3-(1H-imidazol-4yl)-propionic acid + NH3 + H2O2
-
 ?
1.4.3.11 L-Tyr + O2 + H2O 2.7% of the activity with L-glutamate Streptomyces violascens 3-(4-hydroxyphenyl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.11 L-Tyr + O2 + H2O 2.7% of the activity with L-glutamate Streptomyces violascens H82-N-SY7 3-(4-hydroxyphenyl)-2-oxopropionic acid + NH3 + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.11 5
-
-
 Streptomyces violascens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.11 4 7 pH 4.0: about 75% of maximal activity, pH 7.0: about 80% of maximal activity Streptomyces violascens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.11 3 7 37°C, 1 h, stable Streptomyces violascens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.11 FAD one molecule of noncovalently bound FAD per subunit Streptomyces violascens