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Literature summary extracted from

  • Nguyen, L.T.; Nguyen, K.T.; Kopecky, J.; Nova, P.; Novotna, J.; Behal, V.
    Purification and characterization of a novel valine dehydrogenase from Streptomyces aureofaciens (1995), Biochim. Biophys. Acta, 1251, 186-190.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.8 L-valine VDH synthesis induced by L-valine, but severely repressed by ammonia Kitasatospora aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.8 iodoacetamide VDH2: 1 mM, 50% loss of activity Kitasatospora aureofaciens
1.4.1.8 Mn2+ VDH2: 1 mM, 60% loss of activity Kitasatospora aureofaciens
1.4.1.8 p-hydroxymercuribenzoate VDH2: 0.01 mM, complete inhibition Kitasatospora aureofaciens
1.4.1.23 4-hydroxymercuribenzoate 0.01 mM, 100% inhibition Kitasatospora aureofaciens
1.4.1.23 iodoacetamide 1 mM, 50% inhibition Kitasatospora aureofaciens
1.4.1.23 Mn2+ 1 mM, 60% inhibition Kitasatospora aureofaciens
1.4.1.23 additional information activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.8 0.46
-
2-oxoisovalerate VDH2 Kitasatospora aureofaciens
1.4.1.8 0.75
-
L-valine VDH2 Kitasatospora aureofaciens
1.4.1.8 1.06
-
2-oxo-3-methylvalerate VDH2 Kitasatospora aureofaciens
1.4.1.8 1.2
-
2-oxoisocaproate VDH2 Kitasatospora aureofaciens
1.4.1.8 1.66
-
L-norvaline VDH2 Kitasatospora aureofaciens
1.4.1.8 1.72
-
L-isoleucine VDH2 Kitasatospora aureofaciens
1.4.1.8 1.85
-
L-leucine VDH2 Kitasatospora aureofaciens
1.4.1.8 4.35
-
L-2-aminobutyrate VDH2 Kitasatospora aureofaciens
1.4.1.8 17.2
-
pyruvate VDH2 Kitasatospora aureofaciens
1.4.1.8 22.5
-
L-norleucine VDH2 Kitasatospora aureofaciens
1.4.1.8 117.6
-
L-methionine VDH2 Kitasatospora aureofaciens
1.4.1.8 333.3
-
L-alanine VDH2 Kitasatospora aureofaciens
1.4.1.23 0.42
-
2-Oxoisohexanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 0.46
-
2-oxoisovalerate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 0.75
-
L-valine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 1.06
-
3-methyl-2-oxopentanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 1.2
-
2-oxobutanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 1.66
-
L-norvaline pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 1.72
-
L-isoleucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 1.85
-
L-leucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 4.35
-
L-2-aminobutanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 17.2
-
pyruvate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 22.5
-
L-norleucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.4.1.23 333
-
L-alanine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.8 41000
-
6 * 41000, VDH2, SDS-PAGE Kitasatospora aureofaciens
1.4.1.8 240000
-
VDH2, gel filtration Kitasatospora aureofaciens
1.4.1.23 41000
-
6 * 41000, SDS-PAGE Kitasatospora aureofaciens
1.4.1.23 240000
-
gel filtration Kitasatospora aureofaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.8 L-valine + NAD(P)+ + H2O Kitasatospora aureofaciens VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O Kitasatospora aureofaciens first step in valine catabolism 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O Kitasatospora aureofaciens BS-5 VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O Kitasatospora aureofaciens BS-5 first step in valine catabolism 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.8 Kitasatospora aureofaciens
-
-
-
1.4.1.8 Kitasatospora aureofaciens
-
two isoenzymes: VDH1 and VDH2
-
1.4.1.8 Kitasatospora aureofaciens BS-5
-
-
-
1.4.1.23 Kitasatospora aureofaciens
-
-
-
1.4.1.23 Kitasatospora aureofaciens BS-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.8 VDH2, 52.6fold purification Kitasatospora aureofaciens
1.4.1.23
-
Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.8 45.01
-
NAD+ Kitasatospora aureofaciens
1.4.1.8 64.31
-
oxidative deamination Kitasatospora aureofaciens
1.4.1.8 366.5
-
reductive amination Kitasatospora aureofaciens

Storage Stability

EC Number Storage Stability Organism
1.4.1.8 -80°C, 100 mM Tris-HCl buffer, pH 7.4, 20% v/v glycerol, 2 months, stable Kitasatospora aureofaciens
1.4.1.23 -80°C, presence of 20% glycerol, stable for at least 2 months Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ VDH2: 125% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ VDH2: 125% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 VDH2: reductive amination, 116% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-alanine + H2O + NAD+ VDH2: 4.5% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate r
1.4.1.8 L-alanine + H2O + NAD+ VDH2: 4.5% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH VDH2: reductive amination, 20.8% of the activity with 2-oxoisovalerate r
1.4.1.8 L-isoleucine + H2O + NAD+ VDH2: 34% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
1.4.1.8 L-isoleucine + H2O + NAD+ VDH2: 34% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NADH + NH3 VDH2: reductive amination, 24.5% of the activity with 2-oxoisovalerate r
1.4.1.8 L-leucine + H2O + NAD+ VDH2: 52.8% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
1.4.1.8 L-leucine + H2O + NAD+ VDH2: 52.8% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisocaproate + NADH + NH3 VDH2: reductive amination, 26.5% of the activity with 2-oxoisovalerate r
1.4.1.8 L-methionine + H2O + NAD+ VDH2: 2% of the activity with L-valine Kitasatospora aureofaciens 4-(methylthio)-2-oxobutanoic acid + NH3 + NADH
-
r
1.4.1.8 L-norleucine + H2O + NAD+ VDH2: 18.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxocaproate + NADH + NH3
-
r
1.4.1.8 L-norvaline + H2O + NAD+ VDH2: 100% of the activity with L-valine Kitasatospora aureofaciens 2-oxovalerate + NH3 + NADH
-
r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O first step in valine catabolism Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens BS-5 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens BS-5 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens BS-5 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O VDH is required for utilization of branched chain amino acids, the catabolism appears to be an alternative source of n-butyrate, 2-methylmalonate, and propionate needed for biosynthesis of macrolide and polyether antibiotics Kitasatospora aureofaciens BS-5 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O first step in valine catabolism Kitasatospora aureofaciens BS-5 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.23 2-oxobutanoate + NH3 + NADH + H+ 116% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-2-aminobutanoate + H2O + NAD+
-
r
1.4.1.23 2-oxoisohexanoate + NH3 + NADH + H+ 26.5% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-leucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisovalerate + NH3 + NADH + H+
-
Kitasatospora aureofaciens L-valine + H2O + NAD+
-
r
1.4.1.23 3-methyl-2-oxopentanoate + NH3 + NADH + H+ 24.5% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-isoleucine + H2O + NAD+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 125% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-alanine + H2O + NAD+ 4.5% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+ 34% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+ 52.8% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norleucine + H2O + NAD+ 18.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 100% of the activity with L-valine Kitasatospora aureofaciens 2-oxovalerate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Kitasatospora aureofaciens 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 additional information no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate Kitasatospora aureofaciens ?
-
?
1.4.1.23 pyruvate + H2O + NAD+ 20.8% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-alanine + NH3 + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.8 hexamer 6 * 41000, VDH2, SDS-PAGE Kitasatospora aureofaciens
1.4.1.23 hexamer 6 * 41000, SDS-PAGE Kitasatospora aureofaciens

Synonyms

EC Number Synonyms Comment Organism
1.4.1.23 VDH2
-
Kitasatospora aureofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.8 65
-
-
Kitasatospora aureofaciens
1.4.1.23 65
-
both reduxtive amination and oxidative deamination Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.8 9
-
VDH2: reductive amination of 2-oxoisovalerate, 0.3 M Tris-HCl buffer Kitasatospora aureofaciens
1.4.1.8 10.4
-
VDH2: oxidative deamination of L-valine, 0.3 M glycine-KCl-KOH buffer Kitasatospora aureofaciens
1.4.1.23 9
-
reductive amination Kitasatospora aureofaciens
1.4.1.23 10.4
-
oxidative deamination Kitasatospora aureofaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.8 1,N6-etheno-NAD+ VDH2: 62.4% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.8 3-pyridinealdehyde-NAD+ VDH2: 37.5% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.8 Alpha-NAD+ VDH2: 5% of the activity with NAD+, VDH1: no activity Kitasatospora aureofaciens
1.4.1.8 deamino-NAD+ VDH2: 375.5% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.8 additional information VDH2: not with 3-acetylpyridine-NAD+, compared to VDH1 with 100% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.8 additional information not with NADP+ and NADPH Kitasatospora aureofaciens
1.4.1.8 NAD+ NAD+ required, no use of NADP+ Kitasatospora aureofaciens
1.4.1.8 NAD+ natural cofactor for oxidative deamination Kitasatospora aureofaciens
1.4.1.8 NADH NADH could not be replaced with NADPH for the reductive amination Kitasatospora aureofaciens
1.4.1.8 nicotinamide guanine dinucleotide VDH2: 80% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 1,N6-etheno-NAD+ 62% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 deamino-NAD+ 373% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 additional information no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+ Kitasatospora aureofaciens
1.4.1.23 NAD+
-
Kitasatospora aureofaciens
1.4.1.23 NADH
-
Kitasatospora aureofaciens
1.4.1.23 nicotinamide guanine dinucleotide 80% of the activity with NAD+ Kitasatospora aureofaciens