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Literature summary extracted from

  • Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Flieger, M.; Basarova, G.; Behal, V.
    Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens (1988), J. Bacteriol., 170, 5192-5196.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.23 Hg2+ HgCl2: 0.01 mM, 91% loss of activity Kitasatospora aureofaciens
1.4.1.23 additional information no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil Kitasatospora aureofaciens
1.4.1.23 p-chloromercuribenzoate 0.01 mM, 83% loss of activity Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.23 0.023
-
NADH
-
Kitasatospora aureofaciens
1.4.1.23 0.023
-
NAD+ pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 0.1
-
NAD+
-
Kitasatospora aureofaciens
1.4.1.23 0.8
-
2-oxoisocaproate
-
Kitasatospora aureofaciens
1.4.1.23 0.8
-
2-Oxoisohexanoate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 1.25
-
2-oxoisovalerate
-
Kitasatospora aureofaciens
1.4.1.23 1.3
-
2-oxoisovalerate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 1.5
-
2-oxobutyrate
-
Kitasatospora aureofaciens
1.4.1.23 1.5
-
2-oxobutanoate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 2.5
-
L-valine
-
Kitasatospora aureofaciens
1.4.1.23 2.5
-
L-valine pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 5
-
L-isoleucine
-
Kitasatospora aureofaciens
1.4.1.23 5
-
L-isoleucine pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 5.7
-
L-norvaline
-
Kitasatospora aureofaciens
1.4.1.23 5.7
-
L-norvaline pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 6.3
-
L-leucine
-
Kitasatospora aureofaciens
1.4.1.23 6.3
-
L-leucine pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 6.7
-
2-oxo-3-methylvalerate
-
Kitasatospora aureofaciens
1.4.1.23 6.7
-
3-methyl-2-oxopentanoate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 14.8
-
L-2-aminobutyrate
-
Kitasatospora aureofaciens
1.4.1.23 14.8
-
L-2-aminobutanoate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 15.3
-
pyruvate
-
Kitasatospora aureofaciens
1.4.1.23 15.3
-
pyruvate pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 15.6
-
L-norleucine
-
Kitasatospora aureofaciens
1.4.1.23 15.6
-
L-norleucine pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 18.2
-
NH4+
-
Kitasatospora aureofaciens
1.4.1.23 18.2
-
NH3 pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 333
-
L-alanine pH 10.4, 30°C Kitasatospora aureofaciens
1.4.1.23 333.6
-
L-alanine
-
Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.23 29000
-
4 * 29000, SDS-PAGE Kitasatospora aureofaciens
1.4.1.23 116000
-
equilibrium ultracentrifugation Kitasatospora aureofaciens
1.4.1.23 116000 118000 gel filtration and equilibrium ultracentrifugation Kitasatospora aureofaciens
1.4.1.23 118000
-
gel filtration Kitasatospora aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.23 Kitasatospora aureofaciens
-
-
-
1.4.1.23 Kitasatospora aureofaciens
-
a UV mutant derived from the high-producing strain 84/25
-
1.4.1.23 Kitasatospora aureofaciens 50/137
-
a UV mutant derived from the high-producing strain 84/25
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.23
-
Kitasatospora aureofaciens
1.4.1.23 785fold purification Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.23 additional information
-
-
Kitasatospora aureofaciens

Storage Stability

EC Number Storage Stability Organism
1.4.1.23 -25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity Kitasatospora aureofaciens
1.4.1.23 4°C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.23 2-oxobutanoate + NH3 + NADH + H+ 48.2% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-2-aminobutanoate + H2O + NAD+
-
r
1.4.1.23 2-oxoisohexanoate + NH3 + NADH + H+ 38.1% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-leucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisovalerate + NH3 + NADH + H+
-
Kitasatospora aureofaciens L-valine + H2O + NAD+
-
r
1.4.1.23 3-methyl-2-oxopentanoate + NH3 + NADH + H+ 22.3% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-isoleucine + H2O + NAD+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 16.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 16.5% of the activity with L-valine Kitasatospora aureofaciens 50/137 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-2-aminobutyrate + H2O + NAD+ 16.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 reductive amination: 48.2% of the activity with 2-oxoisovalerate r
1.4.1.23 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Kitasatospora aureofaciens 2-oxobutyrate + NADH + NH3 reductive amination: 48.2% of the activity with 2-oxoisovalerate r
1.4.1.23 L-alanine + H2O + NAD+ very poor substrate: 1.2% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate r
1.4.1.23 L-alanine + H2O + NAD+ very poor substrate: 1.2% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate r
1.4.1.23 L-alanine + H2O + NAD+ 1.9% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+ 46.5% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
1.4.1.23 L-isoleucine + H2O + NAD+ 46.5% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NADH + NH3 reductive amination: 22.3% of the activity with 2-oxoisovalerate r
1.4.1.23 L-isoleucine + H2O + NAD+ 46.5% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+ 36% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
1.4.1.23 L-leucine + H2O + NAD+ 36% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisocaproate + NADH + NH3 reductive amination: 38.1% of the activity with 2-oxoisovalerate r
1.4.1.23 L-leucine + H2O + NAD+ 36% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norleucine + H2O + NAD+ 10.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxocaproate + NADH + NH3
-
r
1.4.1.23 L-norleucine + H2O + NAD+ 10.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 43% of the activity with L-valine Kitasatospora aureofaciens 2-oxovalerate + NH3 + NADH
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 43% of the activity with L-valine Kitasatospora aureofaciens 50/137 2-oxovalerate + NH3 + NADH
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 43.0% of the activity with L-valine Kitasatospora aureofaciens 2-oxovalerate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Kitasatospora aureofaciens 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 2-oxoisovalerate + NH3 + NAD(P)H highest activity with 2-oxoisovalerate r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 50/137 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 50/137 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.23 L-valine + NAD(P)+ + H2O NAD+ Kitasatospora aureofaciens 50/137 2-oxoisovalerate + NH3 + NAD(P)H highest activity with 2-oxoisovalerate r
1.4.1.23 pyruvate + H2O + NAD+ 1.3% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-alanine + NH3 + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.23 tetramer 4 * 29000, SDS-PAGE Kitasatospora aureofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.23 30
-
assay at Kitasatospora aureofaciens
1.4.1.23 65
-
-
Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.23 9
-
reductive amination Kitasatospora aureofaciens
1.4.1.23 9
-
reductive amination of 2-oxoisovalerate, Tris-HCl buffer Kitasatospora aureofaciens
1.4.1.23 10.7
-
oxidative deamination Kitasatospora aureofaciens
1.4.1.23 10.7
-
oxidative deamination of L-valine, glycine-KCl-KOH buffer Kitasatospora aureofaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.23 1,N6-etheno-NAD+ 58.4% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 3-acetylpyridine-NAD+ 100% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 3-pyridinealdehyde-NAD+ 37.4% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 deamino-NAD+ 83.7% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 additional information no cofactor: NADP+ Kitasatospora aureofaciens
1.4.1.23 NAD+
-
Kitasatospora aureofaciens
1.4.1.23 NAD+ NAD+ required, no use of NADP+ Kitasatospora aureofaciens
1.4.1.23 NADH
-
Kitasatospora aureofaciens
1.4.1.23 NADP+ only 8.4% of the activity with NAD+ Kitasatospora aureofaciens
1.4.1.23 nicotinamide guanine dinucleotide 72.3% of the activity with NAD+ Kitasatospora aureofaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.23 Kitasatospora aureofaciens isoelectric focusing
-
5.1